Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions

摘要

Batch fermentation of the recombinant strain WB800-P - . The values of OD (orange curve), extracellular enzyme activity (blue curve), dissolved oxygen (green curve), and pH (light blue curve) were measured during the fermentation process. The averages of three independent experiments together with the corresponding standard deviations are shown for all values of OD and enzyme activity