In order to improve the enzyme activity of cold temperature amylase,the technological conditions were used to optimize Bacillus subtilis to probuce cold temperature amylase by response surface. Based on single factor experiment,the 3 factors affacting enzyme activity were as follows;the best appropriate temperature,the best appropriate pH value and metal ions. The enzyme activity was used as response value to respond to surface optimization and to test the optimized plan. The experiment result showed that the optimized parameters of enzyme activity of cold temperature amylase were: the most appropriate response temperature was 30℃ ,the most appropriate pH value was 6,the metal ions with the strongest activation for enzyme were Ca2+ ,whose concentration was 0. 01 mol/L,under above conditions,the activity of cold temperature amylase was 32. 67 U/mL,which was the good match for the model.%枯草芽孢杆菌可以产低温淀粉酶,为了提高低温淀粉酶的酶活力,采用响应面法优化枯草芽孢杆菌发酵产低温淀粉酶的工艺条件.在单因素试验的基础上,确定对酶活力影响较大的三个因素,即:最适温度、最适pH值、金属离子.以酶活力为响应值,进行响应面法优化,并验证优化方案.试验结果表明,低温淀粉酶酶活的优化参数为:最适反应温度为30℃,最适pH值为6.0,对酶活力激活作用最强的金属离子为Ca2+,浓度为0.01 mol/L,在此条件下,低温淀粉酶活力为32.67 U/mL,与模型拟合度高.
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