Transcription is the first of many biochemical steps that turn the genetic information found in DNA into the proteins responsible for driving cellular processes. In this review, we highlight certain advantages of single-molecule techniques in the study of prokaryotic and eukaryotic transcription, and the specific aspects in which these techniques complement conventional, ensemble-based biochemistry. We focus on recent literature, highlighting ways in which single-molecule methods have provided fresh insights into mechanism. We also present recent technological advances and outline future directions in the field.
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