Detection of BRAF Mutations Using a Fully Automated Platform and Comparison with High Resolution Melting Real-Time Allele Specific Amplification Immunohistochemistry and Next Generation Sequencing Assays for Patients with Metastatic Melanoma

摘要

BackgroundMetastatic melanoma is a severe disease with one of the highest mortality rate in skin diseases. Overall survival has significantly improved with immunotherapy and targeted therapies. Kinase inhibitors targeting BRAF V600 showed promising results. BRAF genotyping is mandatory for the prescription of anti-BRAF therapies.