Intracellular Localization of Endogenous Mouse ABCG1 Is Mimicked by both ABCG1-L550 and ABCG1-P550

摘要

ObjectiveIn a recent article in Arteriosclerosis, Thrombosis and Vascular Biology, it was reported that ABCG1 containing leucine at position 550 (ABCG1-L550) was localized to the plasma membrane, whilst ABCG1-P550 (proline at position 550) was intracellular. As the published data on the sub-cellular localization of ABCG1 are controversial, we performed additional experiments to determine the importance of leucine or proline at amino acid 550.