18F-FDG-labeled red blood cell PET for blood-pool imaging: preclinical evaluation in rats

摘要

BackgroundRed blood cells (RBCs) labeled with single-photon emitters have been clinically used for blood-pool imaging. Although some PET tracers have been introduced for blood-pool imaging, they have not yet been widely used. The present study investigated the feasibility of labeling RBCs with ¹⁸F-2-deoxy-2-fluoro-D-glucose (¹⁸F-FDG) for blood-pool imaging with PET.RBCs isolated from venous blood of rats were washed with glucose-free phosphate-buffered saline and labeled with ¹⁸F-FDG. To optimize labeling efficiency, the effects of glucose deprivation time and incubation (labeling) time with ¹⁸F-FDG were investigated. Post-labeling stability was assessed by calculating the release fraction of radioactivity and identifying the chemical forms of ¹⁸F in the released and intracellular components of ¹⁸F-FDG-labeled RBCs incubated in plasma. Just after intravenous injection of the optimized autologous ¹⁸F-FDG-labeled RBCs, dynamic PET scans were performed to evaluate in vivo imaging in normal rats and intraabdominal bleeding models (temporary and persistent bleeding).