A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation melanoma cell invasion and drug-induced selection of ABCB5-expressing cells

摘要

BackgroundDifferent 3D-cell culture approaches with varying degrees of complexity have been developed to serve as melanoma models for drug testing or mechanistic studies. While these 3D-culture initiatives are already often superior to classical 2D approaches, they are either composed of only melanoma cells or they are so complex that the behavior of individual cell types is hard to understand, and often they are difficult to establish and expensive.