Promotion of endocytosis efficiency through an ATP‐independent mechanism at rat calyx of Held terminals

摘要

Key points class="unordered" style="list-style-type:disc" id="tjp12434-list-0001">At rat calyx of Held terminals, ATP was required not only for slow endocytosis, but also for rapid phase of compensatory endocytosis.An ATP‐independent form of endocytosis was recruited to accelerate membrane retrieval at increased activity and temperature.ATP‐independent endocytosis primarily involved retrieval of pre‐existing membrane, which depended on Ca²⁺ and the activity of neutral sphingomyelinase but not clathrin‐coated pit maturation.ATP‐independent endocytosis represents a non‐canonical mechanism that can efficiently retrieve membrane at physiological conditions without competing for the limited ATP at elevated neuronal activity.