A simple high performance liquid chromatography method for determination of rebamipide in rat urine

摘要

class="kwd-title">Keywords: Rebamipide, HPLC, Extraction, Fluorescence, Urine, Ofloxacin class="head no_bottom_margin" id="idm140604007282224title">AbstractRebamipide is a mucoprotective agent commonly used to prevent nonsteriodal anti-inflammatory drug-induced gastrointenstinal side effects . Human plasma and urine analysis of rebamipide utilizing high performance liquid chromatography (HPLC) have been reported . Recently, we reported on the plasma levels of rebamipide in presense or absence of celecoxib or diclofenac in rats using a modified HPLC method of detection developed by Jeoung et al. . To tailor the method towards use in urinary rebamipide extraction and analysis, the following modifications were made: class="first-line-outdent">

• To compensate for high concentrations of rebamipide found in urine, a new rebamipide stock solution was prepared with a final concentration of 50,000 ng/mL.

• Rat urine calibration standards were obtained within the range of 50–1000 ng/mL and 1000–50,000 ng/mL.

• Plasma samples were replaced with urine samples.