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Genome-wide association study of pre-harvest sprouting resistance inChinese wheat founder parents

机译:全基因组收获前抗性的全基因组关联研究中国小麦创始人的父母

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摘要

Pre-harvest sprouting (PHS) is a major abiotic factor affecting grain weight and quality, and is caused by an early break in seed dormancy. Association mapping (AM) is used to detect correlations between phenotypes and genotypes based on linkage disequilibrium (LD) in wheat breeding programs. We evaluated seed dormancy in 80 Chinese wheat founder parents in five environments and performed a genome-wide association study using 6,057 markers, including 93 simple sequence repeat (SSR), 1,472 diversity array technology (DArT), and 4,492 single nucleotide polymorphism (SNP) markers. The general linear model (GLM) and the mixed linear model (MLM) were used in this study, and two significant markers (tPt-7980 and wPt-6457) were identified. Both markers were located on Chromosome 1B, with wPt-6457 having been identified in a previously reported chromosomal position. The significantly associated loci contain essential information for cloning genes related to resistance to PHS and can be used in wheat breeding programs.
机译:收获前发芽(PHS)是影响谷物重量和品质的主要非生物因素,并且是由种子休眠的早期中断引起的。关联映射(AM)用于基于小麦育种程序中的连锁不平衡(LD)检测表型和基因型之间的相关性。我们在五个环境中评估了80位中国小麦创始人父母的种子休眠状态,并使用6,057个标记进行了全基因组关联研究,其中包括93个简单序列重复(SSR),1,472个多样性阵列技术(DArT)和4,492个单核苷酸多态性(SNP)标记。本研究使用通用线性模型(GLM)和混合线性模型(MLM),并鉴定了两个重要标记(tPt-7980和wPt-6457)。两种标记均位于染色体1B上,wPt-6457已在先前报道的染色体位置被鉴定。显着相关的基因座包含克隆与PHS抗性相关的基因的基本信息,可用于小麦育种计划。

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