逍遥散对慢性应激肝郁脾虚证大鼠行为学及学习记忆能力的影响

摘要

目的:观察慢性束缚应激肝郁脾虚证大鼠行为学与学习记忆能力的变化及逍遥散的调节作用.方法:采用慢性束缚应激方法(每天束缚3 h,连续21 d)制作大鼠模型并用逍遥散进行干预.将45只雄性SD大鼠随机分为正常对照组、模型组、逍遥散组,15只/组.观察各组大鼠一般状态,测量大鼠体重、摄食量、旷场实验行为学指标,并用Y迷宫测试各组大鼠学习记忆能力.结果:1)模型组大鼠在慢性束缚应激初期表现为强烈的反抗、嘶叫,后期表现为神态倦怠、情志抑郁及不再抵抗的行为绝望状态;2)与正常组比较:模型组大鼠体重、摄食量显著下降(P＜0.01);旷场实验模型组大鼠5 min运动总距离减少(P＜0.05),5 min平均移动速度减慢(P＜0.05),中央区停留时间增加(P＜0.05);Y迷宫测试,模型组大鼠正确反应率降低(P＜0.01),全天总反应时间增加(P＜0.01);3)逍遥散对模型组大鼠上述改变均有一定的调节作用.结论:逍遥散对肝郁脾虚证大鼠体重、摄食量及行为学的异常变化具有改善作用,并能提高其学习记忆能力,表现出抗抑郁、焦虑作用.%Objective:To observe the changes of behavior and learning memory ability of chronic immobilization stress liver －stagna－tion and spleen－deficiency syndrome rats and the regulatory effect of Xiaoyao Powder.Methods:A total of 45 male SD rats were randomly divided into 3 groups:normal control group,model group and Xiaoyao powder group.The rat model was established by chronic immobilization stress (daily binding for 3 h,for 21 days) and intervened with Xiaoyao powder.The general state of rats in each group was observed ,and the body mass,food intake,open－field experiment behavioral indicators were measured.Y－maze was used to test the learning and memory ability.Results:The rats in the model group showed strong resistance and screaming at the early stage of chronic immobilization stress ,and later appeared as burnout ,emotional depression and desperate state of behavior of no longer resistance.Compared with normal group :the body mass and food intake of model group rats decreased significantly (P＜0.01); In the open－field experiment,the total length of 5 minutes motion decreased (P＜0.05),and the average moving speed of 5 minutes slowed down (P＜0.05),and the stay period in center zone increased (P＜0.05); In Y－maze test,the rats in model group showed that the correct reaction rate decreased (P＜0.01),and the total reaction time increased throughout the day (P＜0.01).Xiaoyao Powder can regulate the above changes in the model group rats.Conclusion:Xiaoyao Powder can regulate the weight,food intake and abnormal behaviors of liver－qi and spleen deficiency rats.It can also improve the learning and memory abili－ty and the state of depression or anxiety.