糖肾方颗粒不同制备工艺治疗UUO小鼠肾间质纤维化的药效学研究

摘要

Objective:To select the optimal preparation process of sugar－based kidney granules in unilateral ureteral obstruction model mice.Methods:According to macroporous resin enrichment ,alcohol extraction and water extraction methods ,the preparation process of Tangshen Formula Ⅰ ,Ⅱ and Ⅲ were designed.C57BL/6 mice were randomly divided into sham operation group ,model group,route Ⅰ,Ⅱ and Ⅲ groups,and positive drug fosinopril group.Six days after continuous administration ,unilateral ureteral liga－tion was performed.Seven days after surgery ,mice were given serum creatinine and urea nitrogen for 7 days.Semiquantitative analy－sis of renal interstitial fibrosis in each group was performed according to MASSON staining .Renal interstitial cell infiltration scores were evaluated by HE staining.Results:Each route group had a certain improvement on the pathological damage of kidney induced by UUO in mice,all of which significantly improved renal interstitial fibrosis (P＜0.05).Among them,the first and third groups of the process route had better effects.The three routes can significantly improve the serum creatinine and urea nitrogen levels of mice after UUO,and significantly reduce renal interstitial cell infiltration (P＜0.05).Conclusion:The preparation process of Tangshen Formula was determined as the water extraction process.%目的:利用单侧输尿管梗阻模型小鼠筛选糖肾方颗粒的最佳制备工艺.方法:根据大孔树脂富集、醇提、水提方法设计糖肾方制备工艺1、2、3组.选用C57BL/6小鼠,随机分为假手术组,模型组,工艺路线1、2、3组,阳性药福辛普利组,连续给药6 d后,进行单侧输尿管结扎手术.手术后再连续给药7 d,检测小鼠血清肌酐和尿素氮,根据MASSON染色半定量分析各组小鼠的肾间质纤维化,根据HE染色进行肾间质细胞浸润评分.结果:各工艺路线组均对UUO引起的小鼠肾病理损伤有一定的改善作用,均显著改善肾间质纤维化,差异有统计学意义(P＜0.05),其中工艺路线1、3组作用更佳.工艺路线3组能显著改善小鼠UUO后的血肌酐和尿素氮水平,显著减少肾间质细胞浸润,差异有统计学意义(P＜0.05).结论:糖肾方颗粒的制备工艺确定为水提工艺.