银杏蜜环口服溶液改善犬急性心肌缺血的研究

摘要

Objective:To observe the protective effects of Yinxing Mihuan Oral Solution (YXMH) on acute myocardial ischemia injury of dogs.Methods:A total of 36 dogs were randomly divided into model group,diltiazem group (5.0 mg/kg),Gastrodia Tuder Halimasch Powder group(100 mg/kg),Gingkgo biloba leaf extract group (3 mg/kg),low and high doses of YXHM (103 mg/kg;206 mg/kg).The model of acute myocardial infarction was established by ligating anterior descending coronary arteries of dogs.The changes of coronary arteries blood flow volume,blood oxygen content,myocardial consumption of oxygen,myocardial oxygen utilization and myocardial infarct size were observed at different points of time after myocardial ischemia.Results:Compared with the model group,YXHM with low and high doses groups,Gastrodia Tuder Halimasch Powder group and Gingkgo biloba leaf extract group could significantly reduce myocardial infarct size (P ＜ 0.05);Meanwhile,YXHM with high dose could decrease venous oxygen content 30 minutes after myocardial ischemia and increase myocardial consumption of oxygen and myocardial oxygen utilization after 15minutes,30minutes and 60 minutes after myocardial ischemia (P ＜0.05).YXHM with high dose also increased coronary blood flow from 60 minutes to 180 minutes after myocardial ischemia (P ＜ 0.05).Conclusion:YXHM can facilitate myocardial consumption of oxygen,increase coronary blood flow and mycardial oxygen supply,improve myocardial oxygen utilization.Pharmaceutical effect of YXHM is better than Gingkgo biloba leaf extract and Gastrodia Tuder Halimasch Powder.%目的:观察银杏蜜环口服溶液对犬急性心肌缺血的保护作用.方法:36只犬随机分为6组,模型组,合心爽组(5.0 mg/kg);天麻蜜环粉组(100 mg/kg);银杏提取物组(3.0 mg/kg);银杏蜜环口服溶液高剂量组(206 mg/kg)和银杏蜜环口服溶液低剂量组(103 mg/kg).通过结扎犬冠状动脉前降支的方法复制急性心肌缺血模型.观察心肌缺血后不同时间点冠状动脉血流量、血氧含量、心肌耗氧量及心肌氧利用度,心肌梗死范围的变化.结果:与模型组比较,银杏蜜环口服溶液大、小剂量组以及蜜环粉组和银杏提取物组心肌梗死范围明显下降(P＜0.05);同时银杏蜜环口服溶液高剂量组在缺血后30 min静脉血氧含量明显下降(P＜0.05),在缺血后15、30、60 min心肌耗氧量和心肌氧利用度明显增加(P＜0.05),在缺血后60-180 min时冠状动脉血流量明显升高(P＜0.05).结论:银杏蜜环口服溶液可在心肌急性缺血状态下短期内促进心肌氧供给和氧消耗;有效增加冠脉血流量,增加心肌的供血供氧,提高心肌氧利用度.药效作用优于银杏提取物和天麻蜜环粉.