南极海洋芳香烃低温降解微生物的拉曼光谱分析

摘要

Laser tweezers Raman spectroscopy can help with observing and studying individual cells or organdles in a natural state for a relatively long period. In the present experiment, Laser tweezers Raman spectroscopy (LTRS) was used as a tool to report physiological metabolism such as cells growth and nucleic acid, proteins, lipid and glucose of a single active cold-adapted Aromatic hydrocarbons-degradating strains isolated from Antarctic Sea. After the Raman spectrum was collected and analyzed,the findings are as follows: Raman spectrum identified the components of a single cold-adapted Aromatic hydrocarbons-degradating strain and there were more proteins and carbohydrate produced during the Planococcus sp. NJ41 and Shewanella sp. NJ49 growth and degradation; but there was more lipid than the proteins produced during the Pseudoalteromonas sp. NJ289 growth and degradation; the amount of proteins produced by the strains corresponds with the production of degradation rate-limiting enzyme, and was also related to the capacity of low-temperature degradation of aromatic hydrocarbons.%激光镊子拉曼光谱技术可以实现在自然状态下对单个细胞或细胞器较长时间的观察研究.应用激光镊子拉曼光谱技术实时观察南极微生物低温降解芳香烃过程中单个南极细菌的细胞生长和胞内生物大分子的动态变化过程,收集、分析其拉曼光谱,结果发现:单细胞的拉曼光谱反映了其细胞内部的生命物质组成,南极动球菌 NJ41 和希瓦氏菌 NJ49 生长和降解芳香烃过程中产生蛋白类和葡聚糖类物质较多,而假交替单胞菌 NJ289 生长和降解芳香烃过程中产生脂类物质较多,其次才是蛋白类物质;各菌株产生蛋白类物质的多少是与产生的降解限速酶对应的,也是与低温降解芳香烃的能力是直接相关的.