内蒙古农村地区育龄妇女头发中21种元素含量水平及相互作用

摘要

近年来育龄妇女矿物质缺乏的风险增加,特别是农村地区妇女。研究使用电感耦合等离子体原子发射光谱法(ICP-AES)和原子荧光光谱法(AFS)测定内蒙古乌兰察布市兴和县健康育龄妇女头发中21种元素含量(Ca,Mg,Cu,Zn,Fe,Mn,Cr,Ti,B,Co,Mo,Si,V,Ni,Cd,Al,Pb,Ba,Sr,Sn和Se)。结果显示,21个元素的浓度水平明显地不同于文献报道的平均水平。育龄女性存在微量营养素缺乏和有毒元素明显过剩的现象。当地矿产及土壤种类导致镁和铝的含量较高而硒含量较低。通过相关分析和逐步多元回归分析研究了元素间的相互作用,性质相似的金属元素具有显著的相互作用,且最强的是镁与钙、铬与硼、硅与铅、锰与铅、铁与锰。研究地区镉、铅没有显著的相关作用,这可能与工业暴露少有关。同时其他因素也影响元素在人体内的代谢水平。多元回归分析发现：Ca=f(Mg,Se)(Se为负相关,β<0),Fe=f(Cu,Mn, Ti),Zn=f(Ca,Se,Fe,Ni)(Fe,Ni为负相关,β<0),Cu=f(Pb,Fe,Cd),Al=f(Mg,Pb),Se=f(Zn, Cr,Ca)(Ca为负相关,β<0)。这些参数可以用于研究生物体内不同元素之间的关系。聚类分析法进一步对不同元素的来源进行分类,结果表明该地铅暴露主要来源于自然环境。研究结果将为改善农村地区育龄妇女宏观和微量元素水平提供可靠的依据,同时,也有助于制定更有效的提高妇女生殖健康和妊娠结局的策略。%Women of reproductive age are at an increased risk of minerals deficiencies,especially for women in recent years.The obj ective of this study was to investigate the concentrations of 2 1 elements (Ca,Mg,Cu, Zn,Fe,Mn,Cr,Ti,B,Co,Mo,Si,V,Ni,Cd,Al,Pb,Ba,Sr,Sn and Se)in scalp hair of apparently healthy women at childbearing age of Xinghe County,Ulanqab city of Inner Mongolia.The hair samples were determined by inductively coupled plasma-atomic emission spectrometry (ICP-AES)and atomic fluorescence spectrometry (AFS),respectively.The results revealed that the concentration levels of 21 elements were clearly differed from the mean levels reported in the literatures.Micronutrient deficiencies and apparent excess of toxic elements are present in female of childbearing age in studied area.The higher Mg and Al levels may be due to the largest bentonite deposit in North China lay in the study area.The soils in the studied areas had been detected to have low total Se concentration which may account for that 98% participants had Se concent below 0.2μg· g-1 .Inter-element interactions were studied by evaluation of correlation coefficients between two elements,as well as by multiple regression analysis.These elements form divalent ions and their sources are similar were significantly inter-correlated.The strongest relations found between the elements in the hair were as follows:Mg and Ca,Cr and B,Si and Pb,Mn and Pb,Fe and Mn.No correlation was found between Cd and Pb,which is different with other reports.This could be explained by the rarely industrial exposure to these elements.And kinds of geographical environment,dietary habits and other factors in different regions impact human trace element metabolism.Multiple regression analysis presented the results:Ca=f(Mg,Se) (Se are negatively correlated,β<0),Fe=f(Cu,Mn,Ti),Zn=f(Ca,Se,Fe,Ni)(Fe,Ni are negatively correlated,β<0),Cu=f(Pb,Fe,Cd),Al=f(Mg,Pb),Se=f(Zn,Cr,Ca)(Ca are negatively correlated,β<0).These relations can be useful to study the relationships among different elements inside an organism of women.Cluster analysis (CA)was used for further classifying the different sources of elements on the basis of the similarities of their chemical properties.The significantly correlated subcluster of Mn-Pb-Si-Al proved that Pb exposure came from a nature source.The results will provide a reliable basis for improving macro-and mi-croelements status of women in childbearing age in rural area.Meanwhile,this study may help to make more effective strategies to improve the reproductive-aged women’s health and the pregnancy outcome.