The isolation and purification of Sargassum polysaccharides was carried out and the physicochemical property of Sargassum poly-saccharide was analyzed. The effects of S. Polysaccharide on glutathione concentration, nitric oxide production and cell proliferation were investigated in vitro. The results showed that S. Polysaccharide was obtained with a yield of 152.8 g/L (w/w). S. Polysaccharide at concentration of 100, 200 or 400 mg/L could significantly enhance the lymphocyte proliferation (P <0. 05). Hydrogen peroxide at 50 junol/L could induce oxidative stress of splenic lymphocytes in chicken. S. Polysaccharides at 400 mg/L could increase glutathione level significantly when the incubation time were 4 hours or 12 hours (P<0.05) and could decrease the level of nitric oxide in splenic lymphocytes significantly in chicken (P <0.05) at 8 hour. From the findings, it could be concluded that S. Polysaccharides markedly enhance antioxidant activity and may have potential immunomodulatory effect in chicken.%马尾藻具有很高的食用和药用价值,分离提取马尾藻多糖(Sargassum polysaccharides,SP),对其理化性质进行分析；研究马尾藻多糖对氧化应激态鸡脾脏淋巴细胞内谷胱甘肽(GSH)和一氧化氮(NO)分泌水平及淋巴细胞体外增殖的影响.结果表明:马尾藻多糖提取率为152.8 g/L;马尾藻多糖浓度为100、200、400 mg/L时,能显著促进体外培养的鸡脾脏淋巴细胞增殖；H2O2浓度为50 μmol/L时,能诱导体外培养的鸡脾脏淋巴细胞处于氧化应激状态.马尾藻多糖浓度为400 mg/L,与细胞共孵育4h或12h,能显著增加氧化应激态细胞内GSH含量；共孵育培养8h,能显著降低氧化应激态细胞的NO水平.马尾藻多糖能促进鸡脾脏淋巴细胞增殖,并通过调节细胞内GSH和NO水平,提高免疫细胞的抗氧化能力,发挥免疫调节作用.
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