电针对膝骨关节炎MIA模型大鼠软骨组织中Ⅰ型胶原蛋白基因表达的影响

摘要

Objective To investigate whether inhibiting the expression of typeⅠ collagen gene is one of the mechanisms of action of electroacupuncture in improving knee osteoarthritis.Methods Forty male adult SD rats were randomized into normal, model, medication and electroacupuncture group, 10 rats each. A rat model of MIA-induced knee osteoarthritis was made by injecting monomer sodium iodoacetate (MIA) and driving rat movement. After model making, the medication group received an oral gavage of celecoxib dissolved in 10% DMSO and the electroacupuncture group, electroacupuncture at points Zusanli and Yanglingquan. Pain thresholds and the levels of cartilage expression of typeⅠ collagen mRNA were compared between various groups of rats before and after treatment.Results There was a statistically significant difference in pain threshold between the model, medication or electroacupuncture group of rats and the normal group after model making (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in pain threshold between the medication and andelectroacupuncture groups of rats before sacrifice (P>0.05). There was a statistically significant difference in the expression of typeⅠ collagen mRNA between the model, medication or electroacupuncture group of rats and the normal group (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in the expression of typeⅠ collagen mRNA between the electroacupuncture and medication groups of rats before sacrifice (P>0.05).Conclusion The mechanisms of actions of electroacupuncture and medication in treating knee osteoarthritis may be related to inhibiting the expression of typeⅠ collagen mRNA.%目的：观察抑制Ⅰ型胶原蛋白基因表达是否为电针改善膝骨关节炎的作用机制之一。方法将40只成年SD雄性大鼠随机分为正常组、模型组、药物组和电针组，每组10只。采用注射单体碘乙酸钠(MIA)并驱赶大鼠运动来建立膝骨关节炎MIA模型，药物组造模后采用塞来昔布溶入到10%DMSO中进行灌胃，电针组采用电针足三里、阳陵泉治疗。比较各组大鼠治疗前后痛阈及软骨组织内Ⅰ型胶原蛋白mRNA表达水平的变化情况。结果模型组、药物组及电针组大鼠造模后痛阈与正常组比较，差异均具有统计学意义(P＜0.01)。药物组和电针组大鼠处死前痛阈与模型组比较，差异均具有统计学意义(P＜0.01)。药物组大鼠处死前痛阈与电针组比较，差异无统计学意义(P＞0.05)。模型组、药物组及电针组大鼠Ⅰ型胶原蛋白mRNA表达与正常组比较，差异均具有统计学意义(P＜0.01)。药物组和电针组大鼠Ⅰ型胶原蛋白mRNA表达与模型组比较，差异均具有统计学意义(P＜0.01)。电针组大鼠Ⅰ型胶原蛋白 mRNA 表达与药物组比较，差异无统计学意义(P＞0.05)。结论电针与药物对治疗膝骨关节炎的作用机制可能与抑制Ⅰ型胶原蛋白mRNA表达有关。