电针内关穴对心肌肥厚大鼠儿茶酚胺类神经递质的保护作用

摘要

Objective To discuss whether acupuncture at Neiguan (PC 6) inhibits myocardial hypertrophy via monoamine neurotransmitters.Method Sixty female Sprague-Dawley rats were randomized into a control group, a model group, a sham electroacupuncture (EA) group, and an EA group, 15 cases in each group. The rat models of myocardial hypertrophy were developed by subcutaneous injection of Isoprenaline hydrochloride (ISO) for 14 d. The EA group was intervened by EA at Neiguan (PC 6), and the sham EA group was by EA at a sham acupoint [5 mm away lateral to Neiguan (PC 6). The Electrocardiogram (ECG) of each group was recorded, electron microscope was used to observe the microstructure of the rats’ myocardial tissues, and high performance liquid chromatography (HPLC) was adopted to detect the contents of catecholamines in myocardial tissues.Result The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) in the model group and sham EA group were significantly different from that in the control group (P<0.05). The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) of the EA group were significantly different from that of the model group (P<0.05).Conclusion EA at Neiguan (PC 6) can improve myocardial hypertrophy, which is related to the regulation of the topical neurotransmitters; it has provided laboratory evidences for the relationship between Neiguan (PC 6) and heart, i.e. the relationship between acupoints and internal organs.%目的：探讨针刺内关穴是否通过单胺类神经递质抑制心肌肥厚。方法将60只雌性SD大鼠随机分为对照组、模型组、假电针组和电针组,每组15例。采用异丙肾上腺素(isoprenaline hydrochloride, ISO)皮下注射14 d构建大鼠心肌肥厚模型,电针组采用电针内关穴治疗,假电针组采用电针假穴位(内关穴外侧旁开5 mm)治疗。记录各组大鼠心电图,并采用电镜观察各组大鼠心肌组织超微结构,采用高效液相色谱法(high performance liquid chromatography, HPLC)检测各组大鼠心肌组织儿茶酚胺类神经递质含量。结果模型组和假电针组大鼠心率、R-R间期、ST段振幅及心肌组织中儿茶酚胺类神经递质(肾上腺素、去甲肾上腺素、多巴胺)含量与对照组比较,差异均具有统计学意义(P＜0.05)。电针组大鼠心率、R-R间期、ST段振幅及心肌组织中儿茶酚胺类神经递质(肾上腺素、去甲肾上腺素、多巴胺)含量与模型组比较,差异均具有统计学意义(P＜0.05)。结论电针内关穴可以改善心肌肥厚,这与通过调节心脏局部神经递质作用有关,为内关-心脏(经穴-脏腑)相关提供了实验依据。