目的 观察BRL37344对3T3-L1脂肪细胞脂肪分解及瘦素、TNF-cα mRNA表达的影响.方法 将3T3-L1前脂肪细胞分化成熟后,用0、10-9、10-7 mol/L浓度与0、12、24、48 h作用时间的BRL37344进行干预.采用酶法检测甘油释放含量,RT-PCR法检测细胞瘦素、TNF-α mRNA表达.结果 BRL37344可显著增加3T3 -L1脂肪细胞的脂肪分解,10-9、10-7 mol/L的BRL37344作用48 h后,细胞内瘦素mRNA表达量分别降低38％、97％,TNF-αmRNA表达量分别降低65％、130％,呈剂量依赖性；10-1mol/L的BRL37344作用12、24、48 h后,瘦素mRNA表达量分别降低6％、48％、119％,TNF-α mRNA表达量分别降低10％、66％、158％,呈时间依赖性.结论 BRL37344可促进脂肪细胞的脂质分解,抑制瘦素、TNF-α mRNA表达,其抗肥胖作用与促进脂肪分解,改善瘦素、TNF-α抵抗状态有关.%Objective To investigate the effects of BRL37344 on lipolysis metabolism and leptin, TNF-α gene expression in 3T3-L1 adipocytes, and to explore its anti-obesity mechanism at the celluar level. Methods The 3T3-L1 preadipo-cytes were cultured and differentiated into adipocytes, then incubated with BRL37344 at different concentrations (0, 10-9 mol/L, 10-7 mol/L) and durations (0, 12, 24, 48 h). Lipolysis was quantified by glycerol released in the medium which was determined by colorimetric assay. Leptin and TNF-α mRNA expressions were assayed by RT-PCR. Results Lipolysis increased significantly in 3T3-L1 adipocytes after treatment of BRL37344 in a dose- and time-dependent manner. BRL37344 at doses of 10-9 and 10-7mol/L significantly reduced leptin mRNA expression by 34% and 140% , respectively after 48 hours, as well as TNF-α mRNA expression by 65% and 130% , respectively. Leptin mRNA expression reduced by 6% , 48% , 119% , respectively after treatment of 10-7 mol/L BRL37344 for 12, 24, 48 hours, as well as TNF-α mRNA expression by 10% , 66% , 158% , respectively. Conclusion BRL37344 promotes lipolysis and suppresses leptin, TNF-α mRNA expression in 3T3-L1 adipocytes. And its anti-obesity activity of BRL37344 is related with promoting lipolysis, improving resisting statement of leptin, TNF-α.
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