外源性硫化氢对子宫肌瘤细胞增殖和凋亡的影响

摘要

目的 观察外源性硫化氢对入子宫肌瘤细胞增殖和凋亡的影响,并探讨其可能机制.方法 将对数生长期的人子宫肌瘤细胞分为对照组和观察组,观察组加入终浓度分别为10-5、10-4、10-3 mol/L的硫氢化钠(NaHS),分别于培养12、24、48 h后,采用MTT法测算细胞增殖抑制率,用流式细胞术检测细胞凋亡率,采用实时定量PCR和Western blot分别检测p53、Bcl-2 mRNA和蛋白的表达.结果 随着NaHS浓度的增加和作用时间的延长,细胞增殖抑制率逐渐增加(P均＜0.05),细胞凋亡率逐渐增加(P均＜0.05).观察组随着NaHS浓度的增加和作用时间的延长,p53 mRNA和蛋白的表达逐渐增加(P均＜0.05),而Bcl-2 mRNA和蛋白的表达逐渐降低(P均＜0.05).结论 外源性硫化氢可抑制人子宫肌瘤细胞增殖,促进其凋亡,该作用可能与硫化氢上调p53的表达而下调Bcl-2的表达有关.%Objective To observe the effect of exogenous hydrogen sulfide on the proliferation and apoptosis of human uterine leiomyoma cells and to investigate its mechanism.Methods The human uterine leiomyoma cells were randomly divided into the control and observation group.The cells were treated with different concentrations of sodium bisulfide (NaHS) (10-5 mol/L,10-4 mol/L and 10-3 tmoL/L) for 12,24 and 48 hours in the observation group.MTT assay was used to detect the cell proliferation inhibition rate.The apoptosis rates were measured by flow cytometry.The mRNA and protein expressions of p53 and Bcl-2 in human leimyoma cells were measured by Real-time PCR and Western blot.Results With the increase of NaHS concentration and the prolonged time,the cell proliferation inhibition rates were significantly increased (all P ＜ 0.05) and the apoptosis rates were significantly increased (all P ＜ 0.05) in human uterine leiomyoma cells.In the observation group,with the increase of NaHS concentration and the prolonged time,the mRNA and protein expressions of p53 were up-regulated (all P ＜0.05),and the mRNA and protein expressions of Bel-2 were down-regulated (all P ＜ 0.05).Conclusions Exogenous hydrogen sulfide inhibits the cell proliferation and promotes the apoptosis of human uterine leiomyoma cells,whose mechanism may be related to the up-regulation of p53 expression and down-regulation of Bcl-2 expression induced by hydrogen sulfide.