ML-7灌胃对动脉粥样硬化兔肾组织中微量元素含量的影响

摘要

目的：探讨肌球蛋白轻链激酶（MLCK）抑制剂ML-7对动脉粥样硬化（AS）兔肾脏组织微量元素含量的影响。方法将40只新西兰雄性纯种大白兔随机分为3组，分别为ML-7治疗组（17只）、高脂饮食组（13只）、正常对照组（10只），ML-7治疗组给予高脂饲料喂养，第6周开始给予ML-75mg/（kg·d）灌胃，共12周；高脂饮食组给予高脂饲料喂养12周；正常对照组给予基础饲料喂养12周。各组饲养12周时，麻醉后通过颈动脉取血。然后处死，立即取肾脏组织。检测颈动脉血TC水平，采用ICP-AES分析各组肾脏组织中Fe、Mn、Cu、Zn、Se含量。结果ML-7治疗组、高脂饮食组、正常对照组TC水平分别为（21．68±4．69）、（28．33±1．95）、（1．03±0．70）mmol/L，ML-7治疗组、高脂饮食组TC水平均高于正常对照组，ML-7治疗组低于高脂饮食组（P均＜0．05）。与正常对照组比较，高脂饮食组Zn、Mn、Se含量下降，Fe含量升高（P均＜0．05）。与高脂饮食组比较，ML-7治疗组Zn、Mn、Se含量升高，Fe含量下降（P均＜0．05）。结论ML-7能够降低AS兔血清TC水平并提高其肾组织Zn、Mn、Se含量。ML-7可能通过提高肾组织中Zn、Mn、Se含量发挥抗AS作用。%Objective To explore the influence of myosin light chain kinase ( MLCK) inhibitor ML-7 on the contents of Fe, Mn, Cu, Zn, Se, Pb and Cd in rabbits'kidney with atherosclerosis ( AS) .Methods Forty rabbits were randomly divided into 3 groups:ML-7 treatment group (n=17), high-fat diet group (n=13) and normal control group (n=10). Rats in the ML-7 treatment group were fed with high-fat food, from the 6th week on, 5 mg/(kg· d) ML-7 was given via intragastric administration for 12 weeks.High-fat feeding was given to rats in the high-fat diet group for 12 weeks.Rats in the normal control group were given basal feeding for 12 weeks.After feeding for 12 weeks, blood was collected from the carotid of the rabbits in each group after anesthesia .Then kidney tissue were taken immediately after the killing of the rab-bits.TC level in the carotid blood was detected , and the contents of Fe , Mn, Cu, Zn, Se, Pb and Cd in the kidney of each group was analyzed by using ICP-AES.Results TC levels in the ML-7 treatment group , high-fat diet group and nor-mal control group were (21.68 ±4.69), (28.33 ±1.95) and (1.03 ±0.70) mmol/L respectively.TC levels in the ML-7 treatment group and high-fat diet group were higher than that of the normal control group , while TC level in the ML-7 treatment group was lower than that of the high fat diet group (all P<0.05).Compared with the normal control group , the contents of Zn, Mn and Se decreased, while Fe content increased (all P<0.05).Compared with the high-fat diet group, the contents of Zn, Mn and Se increased, while Fe content decreased (all P<0.05).Conclusions ML-7 can decrease TC level in the serum of model rabbits , increase Zn, Mn and Se contents in rabbits'kidney with AS.Meanwhile, ML-7 plays a role of resistance to the AS by increasing the Zn , Mn and Se contents in the kidney .