全身放射线照射对 B16 F10黑色素瘤小鼠的影响

摘要

目的：观察全身放射线照射（ TBI）对B16F10黑色素瘤小鼠移植肿瘤生长及小鼠存活的影响。方法建立C57BL／6小鼠B16F10黑色素瘤移植肿瘤模型，采用不同剂量分别对小鼠进行TBI，观察小鼠移植肿瘤的生长和小鼠的存活情况；检测放疗后小鼠外周血白细胞水平。结果不同剂量TBI对各组小鼠肿瘤面积及存活率无影响（P均＞0．05）。给予7 Gy TBI 10 d后，B16F10荷瘤小鼠外周血白细胞水平下降（P＜0．05）。结论 TBI不影响B16 F10黑色素瘤小鼠移植肿瘤生长及荷瘤小鼠的生存；7 Gy TBI可改变荷瘤小鼠外周血白细胞水平，有利于肿瘤免疫治疗。%Objective To investigate effects of total body irradiation (TBI) on tumor growth and B16F10 melanoma-bearing mice survival.Methods C57BL/6 mice bearing B16-melanoma tumors were irradiated with 0, 5, or 7 Gy total body irradiation ( TBI) , or 7 Gy TBI pus bone marrow transplantation .Tumor areas were measured every 3 days to assess the influences of irradiation treatment on tumor regression .B16-melanoma bearing mice were irradiated with 7 Gy TBI and peripheral blood were harvested at days 1, 3, 5, 7, 9, 11 and 13 after irradiation to test WBC levels .Results TBI with variant dosage on the B 16-melanoma-bearing mice did not influence tumor regression compared with control group ( all P>0.05).WBC levels significantly decreased in the B16F10 melanoma-bearing mice on 10 d after 7 Gy TBI(P<0.05). Conclusion TBI dose do not influence tumor growth and survival of B 16F10 melanoma-bearing mice.Seven Gy TBI can alter WBC levels of peripheral bloods in B 16F10 melanoma-bearing mice, which helps to tumor immunotherapy .