动物皮张基因组 DNA 三种提取方法比较

摘要

With the dry samples of donkey skin as experimental materials,one method was established to extract the whole genomic DNA using SDS -protease K and Chelex -100 combined with Glass Milk for DNA purification technology innovatively.And then,this method was compared to the method for DNA extraction from old and dried skins and the E.Z.N.A Tissue DNA Kit in DNA quality with ultraviolet spectrophotometer detection,agarose gel electrophoresis and PCR detection.The results showed that the method established in this paper was more effective,lower cost and simpler with higher and more stable DNA concentration,which could be utilized better for molecular biological experiments.%以干燥的驴皮张样品为试验材料，将 SDS －蛋白酶 K 法与 Chelex －100法相结合，创新性地引入玻璃奶 DNA 纯化技术，提取全基因组 DNA，并将此方法与前人发表的陈旧皮张 DNA 提取方法和试剂盒提取方法进行比较分析。结果表明，本研究发明方法提取的基因组 DNA 纯度较高，杂质较少，且操作简单，所用试剂、仪器费用较低，能更好地应用于分子生物学实验。