单头西花蓟马总RNA提取方法的比较研究

摘要

TriPure法和RNA提取试剂盒法是目前实验室动植物RNA提取的常用方法.本试验通过对两种不同的RNA提取试剂盒与TriPure法进行比较,发现Trans公司研发的型号为ER501-01的TransZol Up PlusRNA Kit试剂盒适合提取单头西花蓟马总RNA,所得到的RNA纯度和浓度都比较高.该研究结果为通过分子手段研究蓟马等小型昆虫提供理论基础.%TriPure method and RNA extraction kits are the widely used methods for rapid extraction of high quality RNA from plants and animals in labs.In this study,two kinds of RNA extraction kits were compared with the TriPure method.The results showed that the TransZol Up Plus RNA Kit,developed by the Trans Company,was better for extraction of total RNA from individual Frankliniella occidentalis,and the purity and concentration of extracted RNA were both higher.This results would provide theory bases for studying small insects such as Frankliniella occidentalis by molecular technique.