玉米赤霉烯酮污染日粮添加改性蒙脱石对断奶仔猪免疫指标的影响

摘要

[目的]研究1.0mg·kg-1玉米赤霉烯酮(zearalenone,ZEA)污染日粮对断奶仔猪血液学、外周血T淋巴细胞亚群、血清免疫球蛋白和猪瘟抗体效价的影响,同时评价改性蒙脱石的脱毒效应.[方法]将36头试验仔猪按体重随机分为6个处理组(公母各半),仔猪采用试验笼单独饲养;对照组饲喂基础日粮,试验1组在基础日粮的基础上添加1.0g·kg-1改性蒙脱石(商品名:Calibrin-Z),试验2组在基础日粮的基础上添加1.0mg·kg-1ZEA,试验3、4和5组在试验2组的基础上分别添加1.0、2.0和4.0g·kg-1Calibrin-Z.预饲期7d,正式期22d.[结果]与对照组相比,日粮添加1.0mg·kg-1ZEA组能显著降低断奶仔猪血小板、淋巴细胞比率、血红蛋白含量、外周血CD4+和CD8+的比例、血清IgG产量和21d仔猪血清猪瘟抗体水平(P＜0.05),1.0mg·kg-1的ZEA处理组日粮添加2.0-4.0g·kg-1Calibrin-Z能够显著改善ZEA诱导的免疫毒性(P＜0.05);且随日粮Calibrin-Z水平的增加,血小板、淋巴细胞比率、血红蛋白和血清IgG线性升高(P＜0.05).[结论]1.0mg·kg-1的ZEA足以诱导仔猪的免疫毒性,添加2.0-4.0g·kg-1Calibrin-Z具有显著的脱毒效应.%[Objective] The aims of the present study were to investigate the toxicity of zearalenone (ZEA) on haematology, lymphocyte subset in peripheral blood, serum immunoglobulin and swine plague antibody titer production of piglets, and the efficacy of Calibrin-Z in preventing ZEA-induced adverse effects was also evaluated. [Method] The piglets were randomly divided into 6 treatment groups and fed individually in a metabolic cage for 22 days after 7-d adaptation. Piglets in group 1 were fed with basal diet only (Control). Piglets in group 2 were fed with basal diet+1.0 g·kg-1 Calibrin-Z, that in group 3 were fed with basal diet+1.0 mg·kg-1 ZEA, that in group 4 were fed with basal diet +1.0 mg·kg-1 ZEA+ 1.0 g·kg-1 Calibrin-Z, that in group 5 were fed with basal diet + 1.0 mg·kg-1 ZEA + 2.0 g·kg-1 Calibrin-Z, and that in group 6 were fed with basal diet + 1.0 mg·kg-1 ZEA + 4.0 g·kg-1 Calibrin-Z. [Result] Piglets fed ZEA contaminated diet without Calibrin-Z showed significant decreases in platelet, lymphocyte rato, hemoglobin, CD4+ and CD8+ in peripheral blood, serum IgG and swine plague antibody titer production at 21-d when compared with the control (P<0.05). The evident improved effect was observed when addition of 2.0 to 4.0 g Calibrin-Z/kg diet in 1.0 mg·kg-1 ZEA contaminated diet. Platelet, lymphocyte rato, hemoglobin, and serum IgG (P<0.05) showed a linear higher effect with increased Calibrin-Z (P<0.05). [Conclusion] The above findings indicated that ZEA at a level of 1.0 mg·kg-1 was sufficient to induce immunotoxicity of piglets. Addition of 2.0 to 4.0 g Calibrin-Z/kg diet in 1.0 mg·kg-1 ZEA contaminated diet can effectively protect against the detrimental effects of the ZEA feeding. These results may have implications for humans health and animals production consuming ZEA-contaminated food or feed.