系统性红斑狼疮患者外周血单个核细胞超保守区域转录表达水平分析

摘要

目的：探讨超保守区域转录因子与系统性红斑狼疮的相关性。方法：采用T-UCR芯片技术分析了15名系统性红斑狼疮患者和15名健康人群的外周血单个核细胞的超保守区域转录表达水平。结果：比较系统性红斑狼疮组与健康对照组，发现8个T-UCRs （uc.285+, uc.234+, uc.102+, uc.341+, uc.267+, uc.221-, uc.290-and uc.90+）表达水平上调，29个T-UCRs表达水平下调，在表达上调和下调的T-UCRs中，对应的超保守区域uc.186-表达水平最显著（P=3.29,FC=-38.64861），其相关基因为HNRNPH1。结论：通过GO分析后，发现uc.186-可能是系统性红斑狼疮潜在的诊断和预后的生物标志物，HNRNPH1可能是系统性红斑狼疮潜在的关键基因。%Objective:Systemic lupus erythematosus is a systemic autoimmune disease regulated by a variety of genes.T-UCR is an important part of the long non coding RNA transcribed from the 481 extreme conservative regions in the human genome,which will change the expression pattern in many kinds of cancers such as leukemia,liver cancer,colon cancer,neuroblastoma and so on.Method:T-UCR chip technology was used to analyze the expression level of the ultra conservative region of the peripheral blood mononuclear cells in 15 patients with systemic lupus erythematosus and 15 healthy people.Results:Compared the group with systemic lupus erythematosus with the healthy control group,the expression level of 18 T-UCRs(uc.285+,uc.234+,uc. 102+,uc.341+,uc.267+,uc.221-,uc.290-and uc.90+) up-regulated and the expression level of 29 T-UCRs down-regulated,among which the expression of uc.186- was the most signiifcant in the corresponding ultra conserved region (P = 3.29,FC=-38.648 61)and HNRNPH1 was its related gene.Conclusion:GO analysis showed that uc.186- might be a potential biomarker for diagnosis and prognosis of systemic lupus erythematosus,and HNRNPH1 might be the potential key gene of lupus erythematosus system.