4种工业微生物产纤维素酶酶学特性的比较研究

摘要

文章对康宁木霉、绿色木霉、黑曲霉和里氏木霉表达纤维素酶的酶学特性进行了初步研究,结果表明,纤维素酶在pH低于5.0以及温度高于60℃条件下的稳定性较差:Fe2+和Mn2+的存在能够显著提高酶活性:Pb2+和Zn2+的存在严重抑制酶活性.黑曲霉表达纤维素酶活性高,是发酵产纤维素酶的较理想菌株.%Enzymatic characteristics of cellulase of Trichoderma koningii, Trichoderma viride, Aspergillus niger and Trichoderma reesei were investigated. The results showed that the cellulase activity decrease when pH below 5.0 and the temperature above 60 ℃. Fe2+ and Mn2+ can both increased the enzymatic activity, while Pb2+and Zn2+ inhibited the activity greatly. A.niger was considered to be an ideal strain for cellulase production due to the highest activity among the strains.