池塘养殖条件下牙鲆（Paralichthys olivaceus）甲状腺轴和生长轴关键激素的变化规律

摘要

Enzyme-linked immuno sorbent assay was used to measure the serum L-thyroxine (T4), 3,5,3'-triiodo-L-thyronine(T3), growth hormone (GH), and insulin-like growth factor-I (IGF-I) in Japanese flounder (Paralichthys olivaceus) of three ponds. The seasonal variations of body weight and body length were found in present study. Body weight and body length increased constantly as time grows, which were higher in late summer and early autumn compared with other seasons. The average weight of three aquaculture ponds were 1341.77 g, 1198.20 g and 958.93 g and the average length were 54.22 cm, 45.33 cm and 39.40 cm after cultivation for 12 months. The seasonal variations of serum T4, T3, GH and IGF-I were observed. The highest level of the serum T4 was in late summer and the peak of the serum T3 level appeared in early autumn. The highest GH level existed in early summer. The peak level of IGF-I appeared in early autumn. These results revealed the close relationship among the water temperature, body length and body weight growth rate and the serum level of T4 and T3 and T4/T3 increased rapidly, however the potential mechanism need further study.%通过统计学、酶联免疫学等方法研究3个池塘中养殖牙鲆(Paralichthys olivaceus)的生长状况、血清四碘甲状腺素(T4)和三碘甲状腺原氨酸(T3)、生长激素(GH)、类胰岛素生长因子-I(IGF-Ⅰ)和温度的周年变化规律。结果显示，在365 d的养殖过程中，3个池塘牙鲆体重和体长持续增长且体重日增长率和体长日增长率在温度较高的夏末秋初出现较高值。3个池塘牙鲆血清中T3、T4含量变化整体趋势一致，血清T4浓度在夏末出现最高值，T3浓度在秋初出现最高值，滞后于T4。IGF-I水平最高值出现在温度较高的夏末、秋初滞后于GH 最高值夏初。牙鲆血清中T3、T4、GH和IGF-I 4种激素含量变化和生长率变化规律大体一致，呈现明显的周年变化规律。