线粒体辅酶Q改善高脂饮食大鼠肾血流动力学的研究

摘要

Objective To explore the effect of MitoQ on renal hemodynamics in rats fed with high-fat diet.Methods Thirty 8-week-old male SD rats were enrolled and divided into 3 groups randomly.Group N were fed with routine diet,Group H and group HM were fed with high-fat diet.From the 10th week,MitoQ was administered to group HM in the drinking water.At the end of 19th week,All animals underwent Contrast-enhanced ultrasound examination on kidney.The animals were sacrificed for the serum biochemical and renal MDA investigation.Results Compared with group N and group HM,value A(the difference between peak intensity and basement) and value C(related to ascending branch) in group H decreased significantly (P<0.01,respectively).The TTOP (time to peak) and levels of MDA and LDL-c were remarkably increased in group H than that of group N and group HM (P<0.01,respectively).There was no prominent difference in LDL-c between group N and group HM.TC (Total cholesterol) and TG(Triglycerides) in group H and HM were higher than those of group N(P<0.01,respectively).MDA in kidney was significantly increased in group H in comparison with group N and group HM (P<0.01,respectively).Conclusion MitoQ could reduced the level of MDA and protect against the impairment of renal hemodynamics in rats fed with high-fat diet.%目的 探讨线粒体辅酶Q(MitoQ,Mitoquinone)对高脂饮食大鼠肾血流灌注的影响.方法 8周龄雄性SD大鼠30只,随机分成模型组(H组,n=10)、治疗组(HM组,n=10)和对照组(N组,n=10).N组喂食基础饲料,H组、HM组喂食高脂饲料.9周后,HM组自饮水中加入MitoQ.三组大鼠继续喂养10周后进行肾脏声学造影检查,并检测大鼠血生化及肾组织丙二醛(MDA)含量,比较各指标的组间差异.结果 与N组及HM组比较,H组大鼠肾皮质达峰时间(TTOP)延长,增强强度(A)及充盈速度(C)下降(P<0.01),总胆固醇(TC)、三酰甘油(TG)及低密度脂蛋白胆固醇(LDL-c)均明显增高(P<0.01),HM组与H组比较,以上各指标明显改善.H组血清及肾组织丙二醛(MDA)含量明显高于HM组及N组(P<0.01).结论 MitoQ可降低机体内过氧化物含量,改善高脂饮食大鼠肾血流动力学状态.