碳酸锶/丝素蛋白纳米纤维膜的构建及其对骨髓间充质干细胞增殖分化的影响

摘要

目的:构建新型骨引导再生材料碳酸锶/丝素蛋白纳米纤维膜,并初步研究其对于骨髓间充质干细胞增殖分化的影响.方法:通过静电纺丝技术结合生物矿化方法制成碳酸锶/丝素蛋白纳米纤维膜,扫描电镜及X射线衍射分析表征材料的结构及理化特性.将材料与骨髓间充质干细胞共培养,通过cck-8、ALP活性、RT-PCR检测细胞的增殖与骨向分化.结果:本实验制得碳酸锶/丝素蛋白纳米纤维膜,膜外观较矿化前粗糙,电镜显示材料纳米纤维表面出现许多纳米碳酸锶颗粒,分布均匀.骨髓间充质干细胞在碳酸锶/丝素蛋白表面能不断增殖,相较于纯丝素蛋白膜,掺锶的丝素蛋白膜能明显促进骨髓间充质干细胞的碱性磷酸酶活性,同时上调成骨向分化基因的表达;并且碳酸锶/丝素蛋白材料的促成骨向分化作用更为明显.结论:该新型材料具有良好的生物相容性,能更好地促进骨髓间充质干细胞的增殖和骨向分化,可以作为制备引导骨再生膜的潜在替代材料.%Objective: The aim of the study was to fabricate the novel silk fibroin/strontium carbonate nanofibrous membranes (SrCO3-SFM) and explore the effects on the bioactivity of BMSCs.Methods: We fabricated the SrCO3-SFM by electrospinning and biomineralization process.SEM and XRD were used to determine the structure characteristics of SrCO3-SFM.To examine the biocompatibility of SrCO3-SFM, we investigated cell proliferation and differentiation by cck-8, ALP and RT-PCR.Results: The results showed lots of SrCO3 particles, which evenly distributed on the nanofibers.In vitro tests, the SrCO3-SFM promoted the activity of ALP and extracellular matrix mineralization and increased the expression of osteogenic differentiation related genes, such as RUNX2, BSP and OCN.Conclusions: The SrCO3-SFM material has good cell compatibility, promoting mesenchymal stem cell proliferation and osteogenic differentiation.