Objective: To investigate the effect of porous TiO2 modified titanium implant prepared by micro-arc oxidation on the ad-hesion and osteogenic differentiation in bone marrow mesenchymal stem cells(BMSCs).Methods: Micro-arc oxidation technology was used to prepare the coating on the pure titanium surface.The surface morphology and crystal phase of the coating were detected by SEM and XRD.After co-culture of material and BMSCs,the expression of adhesion related protein integrin β1 was detected by immunofluo-rescence staining,in addition,the cytotoxicity of the material was detected by living dead cell staining and MTT.Alkaline phosphatase(ALP)and alizarin red S(ARS)staining assay and RT-PCR were used to evaluate osteogenic differentiation of the BMSCs.Results:The coating of the MAO group treated by micro arc oxidation was microporous TiO2,moreover,the coating not only had no obvious cy-totoxicity,but also promoted the expression of integrin β1 in BMSCs.The results showed that both ALP and ARS staining and quantita-tive of the MAO group was significantly higher than the Ti group which was the untreated group(P＜0.05).The results of RT-PCR showed that the expression level of BMSCs ALP and OSX was significantly increased in MAO group over time.Conclusions: The por-ous TiO2 coating prepared by micro-arc oxidation on pure titanium surface enhanced adhesion and osteogenic differentiation of BMSCs.%目的:探讨微弧氧化制备的多孔TiO2涂层修饰的钛植体对骨髓间充质干细胞(BMSCs)粘附和成骨分化的影响.方法:采用微弧氧化技术在纯钛表面制备涂层,运用扫描电子显微镜(SEM)和X射线衍射仪(XRD)检测涂层表面形貌、晶相.与BMSCs共培养,采用免疫荧光染色检测粘附相关蛋白整合素β1表达,活死细胞染色和MTT法检测材料细胞毒性.碱性磷酸酶(ALP)和茜素红(ARS)染色以及RT-PCR检测涂层对BMSCs成骨分化的影响.结果:采用微弧氧化技术处理后的钛板(MAO组)涂层表面是微米级多孔TiO2,并且涂层无明显细胞毒性,另外还促进了BMSCs中整合素β1表达.ALP和ARS染色及半定量结果显示,与未处理的钛板(Ti组)相比,MAO组BMSCs的ALP和ARS染色结果更深,显著高于Ti组(P＜0.05).RT-PCR结果表明,随着时间的延长,MAO组BMSCs ALP和Osterix表达水平逐渐升高.结论:纯钛表面通过微弧氧化制备的多孔TiO2涂层促进了BMSCs粘附和成骨分化,并且没有明显细胞毒性.
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