Objective; To observe the effects of CS-nano/pcDNA3. 1/GDNF treatment on rats with spinal cord injury(SCI). Methods: One hundred and seventy Wistar rats were randomly divided into 5 groups: A group (laminectomy + SCI + CS-nano/pcDNA3. 1/ GDNF,n = 40), B group (laminectomy+SCI+GDNF,n = 40), C group (laminectomy+SCI + CS-nano,? = 40), D group (laminectomy+SCI,n = 40) , E group (laminectomy, n=10). Spinal cord tissues of rats in each group were harvested on days 5, 10, 20, and 48 h after SCI, and stained with HE, immuno-histochemistry, and TUNEL. Results; HE staining showed extensive spinal cord bleeding, destroyed structure and neuronal necrosis. Immunohistochemistry staining showed that the GDNF positive cells in A group were much more than those in B~D groups after 10,20,or 48 h after SCI. TUNEL staining demonstrated that the positive cells of A group were less than those of B~ D groups(P<0. 01). Conclusion; CS-nano/pcDNA3.1/GDNF treatment can transfer GDNF gene into cells, thus protect the spinal tissue significantly in rats with spinal cord injury.%目的:观察壳聚糖纳米粒子胶质细胞源性神经营养因子基因复合体(CS-nano/pcD-NA3.1/GDNF)对脊髓损伤(SCI)的治疗作用.方法:Wistar大鼠170只,随机分为5组:A组(椎板切除+SCI-CS-nano/pcDNA3.1/GDNF,n=40),B组(椎板切除+SCI+GDNF基因治疗,n=40),C组(椎板切除+SCI+壳聚糖纳米粒子,n=40),D组(椎板切除+SCI,n=40),E组(椎板切除,n=10),分别进行相应处理,于SCI后5、10、20、48h取材后进行HE染色、免疫组化、TUNEL染色.结果:HE染色显示SCI后广泛出血,脊髓结构破坏,神经元死亡;免疫组化染色显示SCI后10、20、48h,A组GDNF阳性细胞数高于B-D组;TUNEL染色显示A组阳性细胞明显较B,C,D组少(P＜O.01).结论:应用CS-nano/pcDNA3.1/GDNF治疗SCI,可以有效地将GDNF基因通过纳米粒子转移入细胞内并进行高效表达,对受损的脊髓组织产生保护作用.
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