结构DNA纳米技术:利用链霉亲和素-生物素相互作用增强DNA纳米结构的稳定性

摘要

Rational design and assembly of nanometer-size objects have been a major goal of nanotechnology and precision engineering.The predictability of duplex interactions and helix geometry of DNA make it an excellent building block for the construction of nanometer-scale structures.DNA nanostructures are prone to dissociation as a result of the change of solution condition.A streptavidinbiotin complexation unit was introduced into the DNA nanostructure to improve its stability.Both the gel test and the melting temperature test prove that the stability of DNA nanostructures can be improved after streptavidin-biotin complexation.This approach is broadly applicable to solving similar problems in structural DNA nanotechnology.%实现纳米尺寸物体的合理设计与组装是纳米技术与精密工程的主要目标之一.DNA因其双链相互作用和螺旋几何构型的可预测性而使其成为构建纳米尺度结构的优秀建筑基元.DNA纳米结构在溶液状况改变时易分解.为提高DNA纳米结构的稳定性,一个链霉亲和素-生物素复合单元被引入到该纳米结构中.凝胶测试与熔点测试均证实链霉亲和素-生物素复合有助于提高DNA纳米结构的稳定性.该方法可广泛用于解决结构DNA纳米技术中的类似问题.