不同磷源对金龟子绿僵菌CQMa102生物量及生成胞外磷酸酶的影响

摘要

Metarhizium anisopliae is an important entomopathogenic fungus which is common and widely distributed in soil throughout the world.Some reports suggested that its extracellular phosphatase might play a pivotal role in invading and killing the hosts.This study explored the effect of different phosphorus sources on biomass growth and extracellular phosphatase biosynthesis of M.anisopliae by using shaking flask methods.Those evaluated phosphorus sources are including inorganic phosphorus (KH2PO4),simple organic phosphorus (phytate sodium and C6H5Na2PO4·2H2O) and protein phosphorus (casein).The results indicated that,in terms of biomass growth,production of acidic phosphatase and extracellular protein,casein yielded the best result,followed by KH2PO4 and C6H5Na2PO4·2H2O,wherein phytate sodium came at last.For purification of the extracellular protein phosphatase of M.anisopliae,the optimal phosphorus source is C6H5Na2PO4·2H2O,where there was the highest specific activity of protein phosphatase.%金龟子绿僵菌Metarhizium anisopliae是一种广泛分布于世界各地土壤中的重要的昆虫病原真菌.已有研究表明,胞外磷酸酶在绿僵菌侵染并致死寄主过程中发挥了重要作用.利用摇瓶培养方法探究了无机磷(KH2PO4)、简单有机磷(植酸钠、磷酸苯二钠)和蛋白有机磷(酪蛋白)为单独磷源条件下,绿僵菌生物量、产胞外酸性磷酸酶以及酪氨酸蛋白磷酸酶、丝/苏氨酸蛋白磷酸酶活性的变化.实验结果显示:加入酪蛋白的培养基,最有利于绿僵菌生长、胞外蛋白的分泌和产酸性磷酸酶；其次为加入KH2PO4和磷酸苯二钠的培养基；加入植酸钠的培养基不利于绿僵菌的生长代谢.然而,加入磷酸苯二钠的培养基,最有利于酪氨酸蛋白磷酸酶、丝/苏氨酸蛋白磷酸酶的分离纯化.