食管鳞状细胞癌组织Raf激酶抑制蛋白和NF-κBp65表达及临床意义

摘要

Objective It is to investigate the expressions of Raf kinase inhibitor protein ( RKIP ) and nuclear factor kappa B p65 ( NF - KBp65 ) in esophageal squamous cell carcinoma ( ESCC ), and explore the correlation between them and their association with clinical pathologic factors of the disease. Methods RKIP and NF - xBp65 were detected by immunohistochemi-cal method in 77 cases of ESCC tissues and 77 cases of adjacent cancerous tissues. The relationship among the expressions of RKIP, NF - xBp65 and ESCC were analyzed. Results The expression of RKIP in ESCC tissues was lower than that in normal adjacent cancerous tissues, while that of NF - xBp65 in ESCC tissues was higher than that in normal adjacent cancerous tissues; the difference was significant ( P <0.01 ). The expression of RKIP in ESCC was related to with or without lymph node metastasis ( P <0.01 ); the expression of NF - K B p65 in ESCC was associated with clinical staging, with or without lymph node metastasis and cell differentiation of the cancer ( P < 0. 05 or 0. 01 ). Conclusion Low or loss of RKIP expression is closely related with the development and progression of ESCC, which facilitates the invasion and metastasis of the disease, probably, by up-regulating NF - xBp65 expression.%目的 探讨食管鳞状细胞癌组织中Raf激酶抑制蛋白(RKIP)和核因子κBp65(NF-κBp65)的表达,及其与食管鳞状细胞癌各临床病理因素之间的关系.方法 用免疫组织化学法检测77例食管鳞状细胞癌和77例癌旁组织的RKIP和NF-κBp65表达,并分析其与食管鳞状细胞癌临床病理学特征关系.结果 食管鳞状细胞癌组织中RKIP的表达低于正常癌旁组织,而NF-κBp65的表达则高于癌旁组织,二者比较有显著性差异(P<0.01 );RKIP在食管鳞状细胞癌组织中的表达与有无淋巴结转移有关(P<0.05).NF-κBp65在食管鳞状细胞癌组织中的表达与临床分期、有无淋巴结转移及肿瘤分化程度有关(P<0.05或0.01).食管鳞状细胞癌组织中RKIP与NF-κBp65的表达呈负相关(P<0.05).结论 RKIP表达减少或缺失与食管鳞状细胞癌的发生、发展密切相关,RKIP表达减少或缺失可能通过上调NF-κBp65的表达促进食管鳞状细胞癌的侵袭和转移.