增殖诱导配体在大肠癌细胞株上的表达、定位及意义

摘要

目的 了解增殖诱导配体(APRIL)在大肠癌细胞株上的表达、定位及意义.方法 分别采用RT-PCR和Western Blotting技术检测APRIL在大肠癌细胞株SW620、LOVO、HCTll6、HT29及SW480上的表达,同时采用A11P法检测这大肠癌细胞株对5-FU的耐药性;以LOVO细胞为研究对象,用细胞免疫荧光技术分析APRIL蛋白的细胞定位情况.结果 RT-PCR和Western-blotting检测显示,APRIL在不同大肠癌细胞株中存在差异性表达,在LOVO、HCTIl6相对高表达,而在SW480、SW620、HT29相对低表达,其表达水平与5-FU耐药性呈正相关.激光共聚焦显微镜观察发现APRIL蛋白在LOVO细胞中主要定位于细胞浆,胞核中少见.结论 APRIL在大肠癌细胞株上有差异表达,其表达水平与大肠癌细胞株的5-FU耐药性有相关性.APRIL主要定位于胞浆.%Objective To investigate the cellular expression and localization of APRIL in colorectal cancer cell lines and their significance. Methods The cellular expression of APRIL mRNA and protein in five colorectal cancer cell lines LOVO, HCT116, SW480. SW620 and HT29. were determined by RT-PCR and Western Bloaing, respectively, and their 5-FU resistance was measured by ATP method. The cellular localization of APRIL protein in colorectal cancer cell line LOVO was checked by fluorescence immunocytochemistry.Results Both the expression degree of APRIL mRNA and protein were different in five colorectal cancer cell lines. They were highly expressed in LOVO and HCT116, and lowly expressed in SW480, SW 620 and HT29,and their expression degrees were positively related to 5-FU resistance. It was showed by fluorescence immunocytochemistry technique that the APRIL protein in LOVO cell line was mainly located in cytoplasm, and litte in nuclear. Conclusion The expression degree of APRIL was different in colorectal cancer cell lines, and positively related to 5-FU resistance. APRIL was mainly located in cytoplasm of colorectal cancer cell line.