Extraction Technology and Content Determination of Total Flavonoids from Traditional Chinese Medicine Yipichou

摘要

[Objectives]The research aimed to optimize extraction technology of total flavonoids from traditional Chinese medicine Yipichou,and establish methods of extraction and content determination of its medicinal materials.[Methods]Using reflux extraction method,total flavonoids of Yipichou were extracted.Via single-factor test,influence of extraction method,extraction solvent concentration,extraction volume and extraction time on content of total flavonoids from Yipichou was inspected.Using orthogonal test,extraction conditions were optimized,and optimal extraction technology of total flavonoids from Yipichou was obtained.[Results]The optimal extraction process of total flavonoids from Yipichou was as below:70%of methanol,1∶40 of solid-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]In the test,total flavonoids were extracted from Yipichou,with better repeatability,and stable and feasible method,which could provide certain scientific basis for studying quality standard of this medicinal material.