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药用植物：英文版

药用植物：英文版

本刊报道内容涉及中草药生药炮制、药用植物化学成分分析及有效成分提取、药用机理研究、临床应用、野生资源调查及栽培驯化等方面。
  • 发文量：524
  • 被引量：23
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  • 开始收录时间：2017(1)
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  • 创刊时间：2010
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    国际刊号/ISSN：2152-3924
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药用植物：英文版 >2023年第6期

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  • 摘要：[Objectives]To study the protective and recovery effects of Yupingfeng polysaccharides(YPF-P)on spleen tissue structure of acute and chronic immunocompromised mice.[Methods]First,90 mice in the protective group were injected with cyclophosphamide to replicate the chronic immunosuppressive model,and observe the protective effects of different doses of YPF-P on the spleen;120 mice in the recovery group were injected with cyclophosphamide continuously to replicate the acute immune injury model,to observe the effects of YPF-P on spleen recovery.Besides,YPF-P positive control group was established to observe its effects on the spleen structure and function of normal mice.Testing indicators include spleen morphology and tissue structure,body mass,and spleen index.[Results]In the protective group and the recovery group,the spleen volume of the mice with different YPF-P doses increased significantly,and lymphocytes proliferated,showing obvious structural compensation and repair,and showing a dose-effect relationship.In the positive control group,the spleen function of the normal mice was significantly enhanced,and the spleen lymphoid tissue showed significant orderly proliferation.[Conclusions]Yupingfeng polysaccharides can effectively protect the function and structure of spleen,improve acute and chronic immune damage induced by cyclophosphamide,greatly promote the recovery of spleen structure and function after immune injury,and also can significant enhance the spleen structure and function of normal mice.
  • 摘要：[Objectives]To optimize the extraction process of polysaccharides from leaves of Dendrobium officinale.[Methods]The polysaccharides from D.officinale were extracted by internal boiling method.The extraction rate of polysaccharides from D.officinale was studied.Based on the single factor experiment,the L 9(3 3)orthogonal experiment was designed to optimize the extraction process of polysaccharides from D.officinale.[Results]The optimum process for extracting polysaccharides from D.officinale by internal boiling method was as follows:1.00 g of D.officinale powder was desorbed with 6.0 mL of 20% ethanol for 20 min,35.0 mL of 90℃ water was rapidly added,and the extraction time was 5 min.[Conclusions]Under this condition,the extraction rate of polysaccharides from D.officinale was 12.31%,which was 3.75 percentage points higher than that of traditional reflux extraction.
  • 摘要：[Objectives]To determine the content of oleanolic acid in 10 batches of Achyranthes aspera L.from different production areas,and to establish a high performance liquid chromatography(HPLC)method for the determination of oleanolic acid in A.aspera.[Methods]Agilent C 18 liquid chromatography column(250 mm×4.6 mm,5 μm)was used for gradient elution with acetonitrile∶water=61∶39 as the mobile phase.The flow rate was 1 mL/min,the detection wavelength was 210 nm,and the column temperature was 35℃.[Results]The oleanolic acid injection volume showed a good linear relationship with the peak area in the range of 0.382-7.640 μg.The linear equation of oleanolic acid was:A=530.76C,R=1.000 0;the range of sample recovery rate was 96.03%-102.73%,and the RSD value was 2.16%(n=9).The content of the oleanolic acid was the highest in A.aspera produced in Guilin City of Guangxi(0.92%),and the lowest oleanolic acid content was in sample of Sitang in Nanning City of Guangxi(0.27%).It is recommended that the oleanolic acid content of A.aspera should not be lower than 0.20%.[Conclusions]The HPLC method is accurate,reliable,easy to operate,and has good resolution.It is suitable for the determination of the content of oleic acid in A.aspera.It can provide reference for the quality control and standard drafting of A.aspera.
  • 摘要：[Objectives]To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology(RSM).[Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology.[Results]The optimal culture conditions:rooting medium 1/2 MS+6-BA 0.24 mg/L+mashed banana 87.63 g/L+potato 89.30 g/L+NAA 0.52 mg/L+sucrose 20.0 g/L+activated carbon 4.0 g/L+agar 7.0 g/L,pH 5.8,and light intensity 2000 Lx.[Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D.officinale.
  • 摘要：[Objectives]This study aimed to explore the effects of moxibustion at the Yanglingquan and Yinlingquan points on thickness of the left foot and synovial tissue inflammation of collagen-induced arthritis(CIA)rats,and to explore the therapeutic effect of moxibustion at complementary acupoints on rheumatoid arthritis(RA).[Methods]Total 32 Wistar rats were selected.Among them,8 rats were selected randomly using the randomized digital method as the blank control(K),and the remaining were subjected to modeling.After the successful establishment of the CIA model,the 24 rats were randomly and evenly divided into model group(M),Yanglingquan and Yinlingquan group(AB)and Shenshu and Zusanli group(CD).The rats in the moxibustion groups were subjected to bilateral synchronous moxibustion once every two days(2 mg refined moxa/cone,5 cones/(point·d)),for total 10 times.The left plantar thickness of each rat was measured before modeling,after modeling,and after the last intervention,respectively.After the last intervention,the situation of synovial tissue inflammation in each rat of the treatment group was inspected.[Results]Compared with that of the control group,the left foot thickness of each group of rats that were modeled all increased significantly(P<0.05).And the synovial tissue showed different degrees of inflammatory infiltration,fibrous hyperplasia,and angiogenes.After the intervention ended,compared with that in the M group,the left foot thickness in the AB and CD treatment groups reduced significantly(P<0.05).The inflammatory infiltration,fibrous hyperplasia,and angiogenes of the synovial tissue decreased.Between the two moxibustion groups,the thickness of the left foot in group AB decreased compared with group CD(P<0.05).The inflammatory infiltration,fibrous hyperplasia and angiogenes in group AB decreased compared with those in group CD.[Conclusions]Moxibustion at the complementary acupoints of Yanglingquan and Yinlingquan,Shenshu and Zusanli both showed good improvement effect on CIA rats.In terms of left foot thickness and synovial tissue inflammation,the treatment group AB was superior to the group CD,and the specific mechanism needs further study.
  • 摘要：[Objectives]To evaluate the effects of Huanglian Jiedu Decoration(HLJD)on the treatment of rat gingivitis with excess internal heat through injecting lipopolysaccharide(LPS)in tooth-supporting tissues.[Methods]Gingival inflammation was established by LPS injection.Male SD rats were randomly divided into 7 groups:normal control group(no treatment group),solvent control group,model group(LPS injection),HLJD low dose group(0.25 g/kg),HLJD medium dose group(0.50 g/kg),HLJD high dose group(1.00 g/kg),and metronidazole and fenbufen capsule(MFC)group.Water intake and urine volume were observed through metabolic cage feeding;gingival tissue protein expressions were detected by LC-ESI-MS/MS method;oxidative stress-related molecules such as total superoxide dismutase(T-SOD)and hydrogen peroxidase catalase(CAT)were detected by the biochemical kit combined with a microplate reader;expressions of TSC related with energy metabolism-related signaling pathway were detected by western blotting.[Results]When rat gingivitis was injected by LPS,both water intake and urine volume were decreased,the levels of T-SOD and CAT were decreased,and the expressions of fructose-bisphosphate aldolase C,triosephosphate isomerase 1 and TSC2 were increased.Huanglian Jiedu Decoration can increase water intake and urine volume,increase the levels of T-SOD and CAT,reduce the expressions of fructose-bisphosphate aldolase C and triosephosphate isomerase 1,and inhibit the expression of energy metabolism-related pathway molecule TSC2.[Conclusions]Huanglian Jiedu Decoration can treat rat gingivitis through inhibiting energy metabolism and oxidative stress such as TSC signal pathway and glycolysis related molecules.
  • 摘要：[Objectives]The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test.[Methods]Phenol-sulfuric acid method was used to determine polysaccharide s content.Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio(A),ultrasonic extraction temperature(B),ultrasonic power(C)and ultrasonic time(D)on extraction rate of O.benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected.[Results]The influence order of solid-liquid ratio(A),ultrasonic extraction temperature(B),ultrasonic power(C)and ultrasonic time(D)on extraction rate of O.benghalensis polysaccharide was A >D >B >C.Via the orthogonal test,it was determined that the optimal extraction condition of O.benghalensis polysaccharide was as below:solid-liquid ratio 1∶25(g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min.Under the condition,extraction rate of O.benghalensis polysaccharide was 9.80%.[Conclusions]The process could provide the reference basis for extracting and producing O.benghalensis polysaccharides.
  • 摘要：[Objectives]To explore the optimal extraction process and the antioxidant activity of flavonoidsof Cacumen platycladi.[Methods]FCPwas obtained from C.platycladi by a new set extraction process of microwave-assisted extraction,freeze thawing,superfine crushingand freeze drying technology and purification.On the basis of single-factor tests,the quadratic regression parasitism model was established by the extraction amount of total flavonoids with microwave power,alcohol concentration,and liquid-to-solid ratio through the response surface methodology.[Results]The optimal extraction conditions were microwave power of 757 W,alcohol concentration of 69%,liquid-to-solid ratio of 21∶1,with the optimal extraction yield of 15.302 9 mg/g.Under the real conditions(microwave power of 800 W,alcohol concentration of 69%,liquid-to-solid ratio of 21∶1)in our laboratory,the maximum yield of total flavonoids was 15.9 mg/g,the results fitwell with the prediction of quadratic regression parasitism model.The good antioxidant activity has been determined byscavenging effect of FCP on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and superoxide anion free radical with the scavenging yield of 70.2% and 68.8% respectively.[Conclusions]All results established a good foundation for furtherdevelopment of C.platycladi products.
  • 摘要：[Objectives]The research aimed to study the effects of applying biofertilizer instead of paclobutrazol on the content of flavonoids in Ophiopogon japonicas and determine its quality characteristics,in order to provide the scientific basis for the quality control of GAP and the comprehensive utilization of resources.[Methods]O.japonicas collected in Lingxing Town and Huayuan Town of Sichuan Province was divided into three groups:blank control group,paclobutrazol group and biofertilizer group.Root tuber of O.japonicas was obtained,and the enzyme was killed by the microwave,and then it was dried in 60℃.The content of flavonoids in root tuber of O.japonicus was determined by ultraviolet spectrophotometry.Then,SPSS19.0 was used to analyze the results.[Results]The average contents of flavonoids in different treatments were as follows:0.184%-0.198% in control group;0.283%-0.330% in the paclobutrazol group;0.404%-0.428% in the biofertilizer group.Biological bacterial fertilizer had a significant role in promoting the synthetic accumulation of homoisoflavonoids in O.japonicus(P<0.001^**).[Conclusions]The application of biological fertilizer can not only significantly improve the content of homoisoflavonoids in root ruber of O.japonicus,but also significantly optimize the micro ecological environment of soil.Therefore,biofertilizer should be widely used to gradually replace paclobutrazol in the main production areas of O.japonicas.
  • 摘要：[Objectives]To explore the clinical efficacy of graphene waist protector on dysmenorrhea of cold-damp stagnation type.[Methods]150 patients with cold-damp stagnation type were randomly divided into 3 groups:50 cases in the experimental group(using graphene waist protector),49 cases in the blank group(using the waist protector for removing the heating plate)and 51 cases in the control group(taking the Tongjingbao granules).After intervening for 3 menstrual cycles,visual analogue scale and the COX menstrual symptom scale were used to evaluate the clinical efficacy of graphene waist protector.[Results]The clinical effective rate of dysmenorrhea in experimental group was 64.00%,while that of control group was 72.54%.With the effective rate of symptoms,the test group was 56.00% and the control group was 64.70%;VAS dysmenorrhea visual score,duration of dysmenorrhea,severity of COX dysmenorrhea and duration of symptoms after intervention were significantly decreased in both experimental group and control group,the differences were statistically significant(P0.05).[Conclusions]Graphene belt of experimental group and Tongjingbao granules of control group both have therapeutic effects on dysmenorrhea of cold-damp stagnation type.Graphene waist protector can relieve the pain and its accompanying symptoms in patients with primary dysmenorrhea of cold-damp stagnation type.
  • 摘要：[Objectives]To make a systematic evaluation of the clinical efficacy and safety of Shenqi Fuzheng Injection in the treatment of colorectal cancer.[Methods]Databases,such as China National Knowledge Infrastructure(CNKI),Wanfang,China biomedical literature service system(CBM),CQVIP,Medline,and Cochrane,were searched for the time before April 2017.The randomized controlled trial(RCT)that met the inclusion criteria was screened.Then,quality evaluation was made based on the modified Jadad scale.Data were extracted and meta-analysis was made with the aid of Review Manager 5.3.[Results]Finally,21 papers met the inclusion criteria,including 2 high quality papers.The average Jadad score was 2.5 points,1 589 patients were included.Meta-analysis results showed that the intervention of the experimental group could improve the quality of life[OR=3.32,95% CI(2.33,4.74),P<0.000 01];improve the short-term efficacy[OR=1.68,95% CI(1.26,2.24),P=0.000 4];reduce the incidence of adverse chemotherapy reactions such as reducing the gastrointestinal reactions[OR=0.33,95% CI(0.25,0.43),P<0.000 01],reducing myelosuppressive reactions[OR=0.33,95% CI(0.24,0.46),P<0.000 01];improve the immune functions,such as increasing CD4/CD8 cell ratio[WMD=1.19,95% CI(0.24,0.41),(P<0.000 01)],and increasing the number of NK cells[WMD=1.72,95% CI(5.62,14.30),(P<0.000 01)].[Conclusions]The clinical effect of Shenqi Fuzheng Injection in the treatment of colorectal cancer is better than that of chemotherapy alone,but it needs more high-quality RCTs to improve the level of research.
  • 摘要：[Objectives]To study the protective effects of self-made Compound Taoren Danshen Decoction(CTDD)on acute liver injury induced by D-galactosamine in rats,and to explore its mechanism.[Methods]An acute liver injury model was established by intraperitoneal injection of 500 mg/kg of D-galactosamine.The ALT,AST,MDA,SOD and GSH-Px in serum,as well as serum TNF-α,IL-1β and IL-6 levels were measured,and liver tissue lesions were observed under microscope.[Results]CTDD can significantly reduce ALT,AST and MDA levels,increase SOD,GSH-Px activity,and significantly reduce serum TNF-α,IL-1β and IL-6 levels,and improve liver tissue lesions.[Conclusions]CTDD has a protective effect on D-galactosamine-induced acute liver injury in rats,and its mechanism may be related to inhibition of oxidative stress and inflammatory reaction.
  • 摘要：[Objectives]To determine the aromatic components of Rosa davurica Pall.[Methods]42 kinds of aromatic components were identified from the flowers of R.davurica by headspace solid phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).The main compounds were alcohols(54.88%)and aldehydes(19.55%).[Results]The top five components with the highest relative content were phenylethyl alcohol(12.69%),geraniol(9.85%),citronellol(8.80%),nerol(7.84%)and 2-n-pentylfuran(7.45%).[Conclusions]Headspace solid phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS)can provide basis for further development and utilization of R.davurica.
  • 摘要：Acanthopanax trifoliatus(L.)Merr is a common traditional Chinese herbal medicine in central and southern regions of China.It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponins and volatile components.Its active extracts have antioxidant,anti-inflammatory,anti-fatigue,and analgesic effects.From the biological characteristics,effective chemical components,extraction methods,cultivation techniques,pharmacological effects,toxicological activities,and product development status,this paper reviewed in detail the research status of A.trifoliatus in China and abroad,to provide a scientific basis for in-depth research and development,and utilization of A.trifoliatus.
  • 摘要：This paper elaborates on the seedling technology,field cultivation technology,field management technology,seed harvesting and seed processing technology in the propagation process of Gentiana macrophylla to provide technical support for artificial breeding of G.macrophylla.
  • 摘要：[Objectives]To explore the effect of Buyanghuanwu decoction on PI3K/AKT signaling pathway and aquaporin AQP4 in cerebral hemorrhage rats and clarify the mechanism to provide clear direction and target for cerebral hemorrhage treatment caused by cerebral edema.[Methods]SD rats were randomly divided into six groups:model group,sham operation group,Buyanghuanwu decoction low,medium and high dose groups,and Ginkgo biloba group.Model group,Buyanghuanwu decoction group,G.biloba group were prepared to be intracerebral hemorrhage rat models by referring to Rosenberg law.While the expression of "polarity" of aquaporin AQP4 was detected by immunofluorescence labeling method,the Evans blue(Evans Blue,EB)content of brain tissue was determined by Spectrophotometry.In addition,the water content of brain tissue was detected by wet and dry weight method.[Results]When compared to the model group,the BuyangHuanwu decoction group,G.biloba group of PI3K and AKT proteins expression increased significantly(P<0.05)and AQP4 in Astrocyte end feet membrane concentrated expression significantly increased(P<0.05),EB content and water content of brain tissue significantly reduced(P<0.05).[Conclusions]The protective mechanisms of Buyanghuanwu decoction on cerebral hemorrhage can work might because it can activate PI3K/AKT signaling pathway,regulate AQP4 "polar" expression,and reduce the permeability of the blood brain barrier and cerebral edema.
  • 摘要：[Objectives]To study the effects of Roudoukou-8 San extract on hydrogen peroxide(H2O2)induced cardiomyocyte injury of rats and explore its action mechanism.[Methods]The cardiomyocytes of neonatal rats were isolated and cultured,and the H2O2 induced cardiomyocyte injury model was established.Methyl thiazolyl tetrazolium(MTT)assay was used to detect the protective effects of Roudoukou-8 San extract on H2O2 induced cardiomyocyte.The effects of Roudoukou-8 San on cardiomyocyte morphology were observed under inverted microscope.The contents of lactate dehydrogenase(LDH),creatine kinase(CK)and aspartate aminotransferase(AST)in cell culture medium were determined by automatic biochemical instrument;the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and nitric oxide(NO)in the cells were detected by kit method.The apoptotic morphology of cardiomyocytes was observed by Hoechst fluorescence staining.Cell apoptosis were measured by Annexin V and PI double staining and flow cytometer.[Results]100 μmol/L of H2O2 acting 2 h could cause about 50% of cardiomyocyte injury.H2O2 model group showed increased cell gap,decreased cell count,cell cytoplasmic vacuoles and other obvious damages.Compared with H2O2 model group,in Roudoukou-8 San extract dose groups,cardiomyocyte morphology showed different degrees of improvement,Roudoukou-8 San extract can significantly reduce the content of LDH,CK and AST in H2O2 injured myocardial cell culture solution,significantly reduce content of MDA and NO in H2O2 injured myocardial cells,increase the SOD activity,and significantly inhibit the apoptosis of H2O2 injured myocardial cells.[Conclusions]Through improving the cell survival status,increasing the cell viability,reducing oxidative stress injury,inhibiting inflammatory responses,and inhibiting apoptosis,Roudoukou-8 San extract can improve the state of H2O2 induced cardiomyocyte injury,so as to protect H2O2 injured myocardial cells.
  • 摘要：[Objectives] The protective mechanism of paeonol on atherosclerotic vessels was investigated by detecting CRP content in the serum and expression of NF-κB p65 in the aorta of atherosclerotic rats. [Methods] An animal model of AS rats was prepared by high-fat diet combined with intraperitoneal injection of VD3. The rats were randomly divided into the normal group,model group,high-dose paeonol group( 120 mg/kg),medium-dose paeonol group( 60 mg/kg),low-dose paeonol group( 30 mg/kg),and simvastatin group( 10 mg/kg) as the positive control group,and drug intervention was continued for 16 weeks. CRP content in the serum was detected by ELISA method,and the expression level of NF-κB p65 was detected by RT-PCR and Western-blot method. [Results]Compared with the normal group,CRP content in the serum and the expression of NF-κB p65 mRNA and protein in the aorta of rats in the model group significantly increased. Compared with the model group,CRP content in the serum dropped obviously,and the expression of NF-κB p65 mRNA and protein in the aorta of rats significantly reduced in the administration groups. Moreover,the improvement of each indicator in the high-dose paeonol group was obviously better than that of the medium-dose and low-dose groups. [Conclusions] Paeonol may protect atherosclerotic vessels by reducing serum CRP content,inhibiting the expression of NF-κB p65 and reducing inflammation.
  • 摘要：[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis.
  • 摘要：Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient content and high cost,limiting the use of D. officinale.Studying the network structure and rate-limiting steps of secondary metabolites of medicinal components of D. officinale,analyzing the secondary metabolic synthesis process,mastering the production rules of its medicinal components and carrying out gene cloning or biosynthesis,etc.are of great significance for the rational development and utilization of D. officinale resources. This paper briefly reviews the progress of the research on the secondary metabolites of D. officinale,including the detection and identification of metabolites and the identification and cloning of key metabolic enzymes.
  • 摘要：[Objectives] To observe the clinical effects of activating blood circulation to resolve blood stasis and glucosamine hydrochloride capsules therapy on post-traumatic knee osteoarthritis treatment,to measure the expression of matrix metalloproteinase in post-traumatic knee osteoarthritis patients before and after treatment,and to explore its relevant molecular mechanisms. [Methods]A total of 60 patients with posttraumatic knee osteoarthritis in early stage in Affiliated Hospital of Shannxi University of Chinese Medicine from January 2015 to December2017 were selected and randomly divided into the control group( n = 30) and the observation group( n = 30). The control group was given oral administration of glucosamine hydrochloride capsules for 6 courses of the treatment. The observation group was using the activating blood circulation to resolve blood stasis therapy for 6 courses of treatment. The recovery of traumatic knee osteoarthritis and the symptom of traditional Chinese medicine( TCM) in 2 groups was compared before and after treatment. The symptoms and the quantitative assessment rating scale of knee osteoarthritis,the pain index of knee and the TCM symptom scores were recorded in both groups. And the synovial fluid and serum of patients were collected to detect the expression of matrix metalloproteinase. [Results]The symptoms and the quantitative assessment rating scale of knee osteoarthritis,the pain index of knee and TCM symptom scores of the 2 groups in the 1 st week,the 12 th week and the 24 th week after the treatment were significantly lower than those before the treatment,and the difference was statistically significant( P 0. 05). The expression level of MMP1 was decreased significantly in the observation group( P 0. 05). [Conclusions]Two treatment methods have been effective in this clinical study. We found that the expression level of MMP3 was decreased significantly after treatment in 2 therapies,which can be clinically applied.
  • 摘要：[Objectives] This study aimed to optimize the medium-pressure preparation process of high-purity ginsenoside Rg1 from triol saponins. [Methods] The reversed-phase C18 chromatographic separation method was used,and the purity and yield of ginsenoside Rg1 were examined as indicators. [Results]The diameter-height ratio of the C18 column was 1∶ 3. 25. Triol saponins of 0. 2 times the volume of the column were loaded with 20% ethanol. At the elution flow rate of 8 BV/h,1,3 and 0. 5 times the volume of the column was eluted with 30%,30%-40% and 40% ethanol,respectively. Crude ginsenoside Rg1 was concentrated,dissolved in 4-time-voume 95% ethanol,added with 0. 5%activated carbon,refluxed for 40 min,and dried to obtain good-quality ginsenoside Rg1. [Conclusions] After purification,ginsenoside Rg1 with purity higher than 99. 5% can be isolated from triol saponins. The medium-pressure preparation process of ginsenoside Rg1 with purity higher than 99. 5% is provided for the first time. It has been proved by many experiments that the process is stable,feasible and reproducible,and can be used for industrial scale-up production.
  • 摘要：[Objectives] To evaluate the phenolic components of Houttuynia cordata Thunb. and their antioxidant activities from different sources. [Methods] Samples of 35 different sources of H. cordata were collected from Guizhou,and polyphenols,total flavonoids,total chlorogenic acid and 7 kinds of flavonoids( xanthol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin,kaempferol and isorhamnetin),and DPPH free radical scavenging rate of the extracts were determined. Relationship between the contents of phenolics and the DPPH free radical scavenging rate of the extracts were analyzed by SPSS software. [Results] There were some significant differences in polyphenols,total flavonoids,total chlorogenic acid and the seven flavonoids between samples of different habitats,and the contents of polyphenols,total flavonoids and total chlorogenic acid increased linearly with altitude. There was a significant positive correlation between polyphenols,total flavonoids and total chlorogenic acid content and DPPH free radical scavenging rate of extracts. The contents of kaempferol-3-O-glucoside,hyperoside,quercitrin,rutin,quercetin kaempferol and isorhamnetin were positively correlated with the DPPH free radical scavenging rates of the extracts,except for the significant correlation with quercitrin. [Conclusions]The experimental results suggest that the antioxidant capacity of H. cordata is derived from the total contribution of the phenolic components,indicating that these components have overall equivalence of antioxidant capacities. This provides a new research idea for solving the key problems of quality control of traditional Chinese medicine.
  • 摘要：Pectinase is a group of enzymes that can decompose complex pectin into small molecules such as galacturonic acid. It is mainly produced by microorganisms by liquid and solid fermentation and immobilized cell method. Its representative strain is Aspergillus niger,and yeast and actinomycete are also high-yield strains for screening pectinase. Pectinase can be used in many fields of life and production,such as clarification of fruit juice and wine,pulping of paper making industry,degumming of textile,extracting of traditional Chinese medicine components,animal feed and other industries,which is economic,green,and efficient and pollution-free. Moreover,pectinase is one of the four major enzymes in the world,and its practical application has very important significance,and the prospect of pectinase application is also important.
  • 摘要：[Objectives] To make a systematic evaluation on the therapeutic efficacy and safety of Yiqi Huoxue traditional Chinese drugs combined with western medicine in treatment of coronary heart disease( CHD) after coronary revascularization. [Methods] The literatures were retrieved from China Knowledge Infrastructure( CNKI),Chinese science and technology journal full-text database( VIP database,VIP),Wanfang Data,the Chinese biomedical Database,Pub Med,Embase,and the Cochrane Library. The retrieval time was set to the creation of the database to January 2017. The randomized controlled trial( RCT) was conducted on the comparison between Yiqi Huoxue Chinese drugs combined with western medicine and the western medicine alone in the treatment of CHD after coronary revascularization. The literature information was extracted and the methodological quality of the included literature was evaluated according to the bias risk assessment tool developed by the Cochrane Collaboration. Meta-analysis was performed with the aid of Rev Man 5. 3 software. Coronary artery restenosis rate,angina pectoris treatment efficiency,left ventricular ejection fraction( LVEF),left ventricular end-diastolic volume( LVEDV),B-type natriuretic peptide( BNP),6-minute walk test( 6 MWT),and adverse reactions were analyzed. [Results] A total of 29 articles were included,a total of2 518 patients,the literature quality was low. Meta-analysis results showed that compared with the treatment by western medicine alone,Yiqi Huoxue Chinese drugs combined with western medicine could further reduce coronary restenosis rate[RR = 0. 45,95% CI( 0. 34,0. 60),P <0. 000 01],improve the angina pectoris treatment efficiency[RR = 1. 13,95% CI( 1. 05,1. 21),P = 0. 000 5],raise LVEF[WMD = 4. 25,95% CI( 3. 46,5. 04),P < 0. 000 01],reduce LVEDV[WMD =-10. 41,95% CI(-17. 88,-2. 95),P = 0. 006],decrease the plasma BNP level[WMD =-32. 32,95% CI(-44. 92,-19. 72),P < 0. 000 01],and increase 6 MWT distance(WMD = 62. 25,95% CI( 21. 71,102. 78),P = 0. 003)[Conclusions]Yiqi Huoxue Chinese drugs combined with western medicine can alleviate the symptoms of angina pectoris,reduce the rate of coronary restenosis,improve heart function and improve exercise capacity,thereby improving clinical efficacy in patients with CHD after coronary revascularization.
  • 摘要：[Objectives] This study aimed to study the effects of Sanguis Draconis flavones( SDF) on the immune regulation of mouse spleen lymphocytes. [Methods]A total of 60 BALB/c male mice were evenly and randomly divided into normal control group,model group,positive group,and high,middle and low-dose SDF groups. The mice in the model group were injected intraperitoneally with cyclophosphamide to establish mouse immunosuppressive model. The mice in the other groups were intragastrically administered at the respective doses,and the body weight was weighed once a week during the administration period. After 30 d of administration,the thymus and spleen indexes,the spleen lymphocyte proliferation ability,the NK cell activity,and the IL-2,IL-4 and INF-γ contents in the culture supernatant were measured. [Results]SDF could significantly increase the thymus and spleen indexes and improve the spleen lymphocyte proliferation ability and NK cell activity of the mice,as well as increasing the IL-2 and INF-γ contents and reducing the IL-4 content in spleen lymphocyte suspension culture. [Conclusions] SDF have good immunoregulatory effect.
  • 摘要：[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the optimal extraction process of Prunellae Spica compound was chosen by using the yield of dry paste and ursolic acid content as evaluation indicators. [Results]The optimal extraction process of Prunellae Spica compound is as follows: solvent dosage was 16 times,and extraction frequency was 3 times,while extraction time was 1 h.[Conclusions]The extraction process of Prunellae Spica compound was stable and feasible,and this study can provide a theoretical basis for further preparation of the compound.
  • 摘要：Jujube is rich in nutrients and functional active ingredients. For example,jujube flavonoids have significant anti-oxidation,anticancer,antibacterial and anti-inflammatory functions. This paper elaborated the advances in the studies about the extraction methods,purification methods,antioxidant properties,stability and antibacterial effects of jujube flavonoids,in order to provide a certain reference for the study of jujube flavonoids.
  • 摘要：[Objectives] To investigate the anti-tumor activity of Sedum bulbiferum Makino in vitro,and establish a HPLC method for determination of quercetin and kaempferol in S. bulbiferum. [Methods] The inhibitory activities of different extracts and total flavonoids of S. bulbiferum on proliferation of three kinds of cancer cells( Hep G2,EC109,SW480) were tested by MTT assay. HPLC method for determination of quercetin and kaempferol in S. bulbiferum was established. [Results]The growth and proliferation of the three kinds of cancer cells were all significantly inhibited by ethyl acetate fraction and total flavonoids isolated from S. bulbiferum. With each extraction concentration increasing,stronger anti-tumor activity was found. The linear ranges of quercetin and kaempferol were 0. 03-0. 36 μg( R = 0. 999 9) and0. 08-0. 96 μg( R = 0. 999 9),and the average recoveries were 98. 90%( RSD = 1. 15%) and 98. 27%( RSD = 1. 70%),respectively.[Conclusions]S. bulbiferum has significant anti-tumor activity in vitro. The HPLC method established was accurate,reproducible,and could be used for quality control of this crude drug.
  • 摘要：[Objectives] The purpose of this study is to dissociate endophytic fungus producing diterpenoids from Torreya fargesii tissue and examines its inhibiting effect on tumor cells. [Methods]Plant endophytes were isolated and purified to study their resistance to Gram-positive( G+) and Gram-negative bacteria( G-). High performance liquid chromatography( HPLC) was used for analysis of the retention time,relative peak area and percentage content of its metabolite. By liquid chromatography-mass spectrometry( HPLC-MS),the material characteristic of the ion pair information of the metabolites was measured. The bacterial strain was also classified. [Results] The results showed that the secondary metabolites produced by the strain BP6 T3 possessed double resistance to G+and G-bacteria. The strain was identified as Penicillium sp by preliminary classification. Through HPLC analysis,the retention time of fermentation extracts was 12. 8 min with almost the same as the standard of taxol. According to the chromatograph,the relative peak area was 12 887. 11,the average relative percentage was about 15. 8%,and the content of taxol analogs in fermentation broth reached 16. 59 mg/L. The material characteristic of the formation of ion fragments of taxane analogues in metabolic extracts was identical to that of the taxol standard determined by HPLC-MS. It can be initially determined that strain BP6 T3 can produce taxane compounds. Taxol substance produced by this strain had obvious inhibitory effect on Hela cells with the concentration increasing. Different precursors had a significant effect on the production of paclitaxel metabolites in this strain. L-phenylalanine was used as the precursor and the yield increased most,with an increase rate of 425. 7%. [Conclusions] The strain is expected to be used for mass production in antitumor drug taxol.

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