CD44基因在卵巢癌中的表达及其与预后的关系

摘要

Objective To investigate the expression of CD44 v6 in ovarian cancer and the relation between its expression and the prognosis. Methods Reverse transcription-polymerase chain reaction ( RT-PCR )was used to investigate the expression of CD44 v6 in 50 cases of ovarian cancer, 10 cases of premalignant tumor of the ovary, 10 cases of benign ovarian tumor and 10 cases of normal tissue. Results ①The positive expression rate of CD44 V6 in ovarian cancer was 95.45% ,higher than that in premalignant tumor of the ovary ( 60% )( P <0.05 ), significantly higher than that in benign ovarian tumor( 20% )and normal tissue( 0% ) ( P <0.01 ). ②The relative amount of CD44 V6 and the epidermis ovarian cancer differentiation degree assumed the inverse proportion, and presented a positive correlation with the staging. ③ The relative amount of CD44V6 and patient's life assumed the inverse proportion. ④The analysis of multivariate Cox proportional hazard regression model indicated that the relative amount of CD44 v6 had independent influence on prognosis of ovarian cancer. Conclusion The positive expression rate of CD44 v6 in ovarian cancer is high,and the relative amount of CD44 v6 mRNA in ovarian is correlated with the histology grade, clinical stage, and lymph node metastasis,which might be the index of the prognosis.%目的 探讨CD44v6在人卵巢癌组织中的表达及其与卵巢癌预后的关系.方法 用半定量反转录聚合酶链反应技术,检测CD44v6在50例卵巢癌组织、10例交界性卵巢肿瘤组织、10例良性卵巢肿瘤组织、10例正常卵巢组织中的表达水平.结果 ①CD44v6在卵巢癌组织中的阳性表达率为95.45%,高于在交界性卵巢肿瘤(60%)的表达(P<0.05),显著高于在良性卵巢肿瘤(20%)及正常卵巢组织中(0%)的表达(P<0.01);②CD44v6 mRNA的相对含量与上皮性卵巢癌的分化程度呈反比,与上皮性卵巢癌的分期呈正比;③CD44v6 mRNA的相对含量与患者的生存时间呈反比;④Cox多因素分析表明CD44v6相对含量的高低是影响术后生存时间的独立预后因素.结论 ①卵巢癌组织中CD44v6阳性表达率高;②CD44v6 mRNA相对含量的高低与组织学分级、临床分期和淋巴结转移均有关;③CD44v6 mRNA相对含量的高低有可能成为预测卵巢癌预后的指标.