基于法布里-珀罗微腔的光微流FRET激光产生

摘要

In order to achieve low-threshold optofluidic fluorescence resonant energy transfer ( FRET ) laser, based on Fabry-Perot ( F-P) microcavity with high quality factor and stability, the generation of optofluidic FRET laser in two types of F-P resonator was studied by using indirect pumping method. Firstly, donor molecules were pumped directly, and then, the excited energies of donor molecules were transferred to acceptor molecules through FRET to realize the indirect optical pumping of the acceptor. Experimental results show that the low energy density threshold of laser pump is 0. 48μJ/mm2 in F-P optofluidic cavity. The results indicate that the detection of low concentration substance can be realized by FRET laser generation in terms of laser detection.%为了实现低阈值光微流荧光共振能量转移(FRET)激光,基于制备的高品质因子、高稳定法布里-珀罗(F-P)微腔,采用间接抽运方法研究了两种F-P谐振腔中光微流FRET激光的产生.直接抽运施主染料,使得施主染料通过FRET的方式把能量传递给受主染料,从而实现受主染料的间接能量抽运.结果表明,在此种抽运方式下,F-P光微流激光腔中实现了0.48μJ/mm2的低激光抽运能量密度阈值;并可通过FRET激光产生的形式实现对低浓度物质的检测.