斑马鱼鳍和鳞片色素细胞的显微观察

摘要

目的 观察斑马鱼鳍和鳞片色素细胞的组成、分布及形态特征.方法 应用光学显微镜对AB品系斑马鱼鳍和鳞片色素细胞的显微结构进行观察.结果 斑马鱼鳍上分布有黑色素细胞和黄色素细胞,未观察到虹彩细胞.根据色素细胞的分布规律,将斑马鱼鳍分为有、无斑马鱼条纹两类.鳞片中分布有黑色素细胞、黄色素细胞和虹彩细胞.黑色素细胞较大,形态上主要分为呈树突状、颜色较浅、体积较大的I类黑色素细胞和呈团状、颜色较深、个体较小的Ⅱ类黑色素细胞.黄色素细胞个体最小,呈黄色或者橙黄色.虹彩细胞较长呈长棒状或梭形,数量最少.结论 斑马鱼体表有黑色素细胞、黄色素细胞和虹彩细胞三种类型色素细胞,但鳍和鳞片在色素细胞组成和分布上存在着差异,鳞片中色素细胞的种类和形态特征较鳍中色素细胞更丰富.%Objective To observe the composition,distribution and morphological characteristics of pigment cells in the fins and scales of zebrafish.Method The microstructure of pigment cells in the fins and scales of AB strain of zebrafish was observed by optical microscope.Results All fins in zebrafish distributed melanophores and xanthophores,without iridophores.According to the distribution of the pigment cells,the zebrafish fins were divided into two types:with or without zebra stripes.There were melanophores,xanthophores and iridophores existed in scales.The melanophores were mainly divided into two types:type Ⅰ was larger,obviously dendritic and light color;type Ⅱ was smaller,nodular,dark color.Xanthophores were the smallest,yellow or orange yellow.Iridophores were a long rod like or fusiform,with smallest number.Conclusion There were three types of pigment cells in the surface of zebrafish including melanophores,xanthophores and iridophores.The composition and distribution of pigment cells in the fins and scales were different.The types and morphological characteristics of pigment cells in the scales were more abundant than that in fins.