Osservazioni di Osso Equino al Microscopio Elettronico a Scansione e all Microtomografia 3D (Scanning Electron Microscope and 3D Microtomography Observations of Equine Bone).

摘要

The aim of this work was to analyze some grafts for bone reconstructions from equine bone tissue, industrially producted, by means of 3D micro-tomography and Scanning Electron Microscope (SEM) images. These samples have been treated by the manufacturer in a suitable way: they have been deantigenized and some of them have been demineralized. In order to evaluate the quality of the deantigenization and demineralization system the analysis was made, to measure the porosity of the samples and to estimate the new micro-tomographyc method. The 3D microtomography technique with Skyscan instrumentation allows to perform structural observations without any particular treatment, alteration or damaging of the sample. Moreover it is possible to perform an acquisition with a 3D reconstruction of the sample, both before and after a test or an implant, for example, into an animal, because this technique does not alter the tested object in any way. Results show that the 3D micro-tomography technique, with Skyscan 1072 instrumentation, could be considered as a valid and effective alternative to the SEM observation.