HPLC法测定番茄红素胶囊中番茄红素的含量

摘要

HPLC method was developed for the determination of lycopene by ODS column (150 mm × 4 .6 mm ,5μm) under the conditions as:acetonitrile‐methyl alcohol‐dichloromethane(60∶25∶15)as the mobile phase ,UV detection wavelength at 472 nm and column temperature at 32 ℃ .Experimental results showed that lycopene has a good linear relation over the range of 0 .2-40 μg/mL(r=0 .999 6)and the average recovery is over 96 .0 % .Lycopene andβ‐carotene are seperated well under the chromatographic conditions .The method proposed is accurate and reliable for determination of lycopene in lycopene capsule with satisfactory results .%建立了用 HPLC法测定番茄红素含量的分析方法，采用ODS色谱柱（150 mm ×4．6 mm ，5μm），流动相为乙腈‐甲醇‐二氯甲烷（60∶25∶15），检测波长472 nm ，柱温32℃。试验结果表明，番茄红素在0．2～40μg／mL范围内线性关系良好（ r＝0．9996），加标回收率大于96．0％，并可同时分离番茄红素和共存的β‐胡萝卜素。该方法准确、可靠，适用于番茄红素胶囊中番茄红素的含量测定。