IL-18及 IL-18结合蛋白、IFN-γ等血清炎性介质在囊型包虫病致 Th1／Th2细胞偏移中的意义

摘要

目的：探讨联合检测血清中白介素18（IL-18）和白介素18结合蛋白（IL-18BP）在囊型包虫病致Th1／Th2细胞偏移发生和发展中的作用。方法以25例健康者为 HC 组，28例囊性包虫病患者为 CE 组，28例囊性包虫病术后患者为 PCE 组。采用酶联免疫吸附法检测3组患者血清 IL-18、干扰素γ（IFN-γ）、白介素2（IL-2）和 IL-18BP、白介素4（IL-4）水平；采用免疫比浊法检测3组患者血清 C 反应蛋白（CRP）、补体3（C3）、补体4（C4）、免疫球蛋白 G（IgG）、免疫球蛋白 M（IgM）、免疫球蛋白 E（IgE）水平。结果CE 组血清 IL-18、IFN-γ、IL-2、C3及C4水平明显低于 HC 组和 PCE 组，差异有统计学意义（P ＜0．05）；CE 组血清 IL-18BP、IL-4、CRP、IgM、IgG 及IgE 水平明显高于 HC 组和 PCE 组，差异有统计学意义（P ＜0．05）。结论血清 IFN-γ、IL-18及 IL-18BP 在肝囊型包虫病所致的 Th1／Th2偏移发生和发展过程中具有协同及相互制约的作用。%Objective To investigate the significance of combined detection of the serum levels of interleukin 18 (IL-18)and interleukin 18 binding protein (IL-18BP)in hepatic cystic echinococcosis caused by Th1/Th2 imbalance. Methods 28 patients with cystic echinococcosis as CE group,28 patients with cystic echinococcosis preoperative surgery as PCE group,25 cases of healthy persons as HC group,Enzyme-linked immunosorbent method to detect the serum IL-18 and IL-18 BP and Th cytokines in each group;Immune turbidimetric method to detect the serum CRP,C3,C4 and IgG,IgM,IgE level in each group.Results (1)Serum IL-18,IFN-γ,IL-2,C3 and C4 concen-trations were remarkably lower in CE compared with HC and PCE group (P <0.05).(2)Serum IL-18BP,IL-4, CRP,IgE,IgM and IgG concentration was higher CE compared with HC and PCE group (P <0.05).Conclusion The imbalance of IL-18 and IL-18BP may one of the possible mechanisim that echinococcus granulosusused to actively modulate and suppress host immune system so that Th1/Th2 immune offset.