巴西橡胶树两个蔗糖合成酶基因的克隆和表达分析

摘要

To investigate the function of sucrose synthase in Hevea brasiliensis growth and development, two genes encoding sucrose synthase, named as HbSS1 and HbSS2, were cloned from H. brasiliensis by RACE-PCR, and their expression patterns were analysed by semi-quantitative RT-PCR. The results showed that the whole length of HbSS1 cDNA was 2864 bp, encoding 806 amino acids;that of HbSS2 cDNA was 2815 bp, encoding 811 amino acids. Both HbSS1 and HbSS2 had typical characteristic of sucrose synthase in plants, containing a conserved phosphorylation site and two conserved domains. The expressions of HbSS1 and HbSS2 were detected in all tissues, their expressions were the highest in leaves and bark, respectively. It was suggested that HbSS1 and HbSS2 might be involved in growth and metabolism of various tissues, and they also had functional differentiation.%为了解蔗糖合成酶在巴西橡胶树(Hevea brasiliensis)生长和发育过程中的功能，利用RACE技术从巴西橡胶树中克隆了蔗糖合成酶基因，并对基因的表达特征进行了分析。结果表明，从巴西橡胶树中克隆了两个蔗糖合成酶基因(HbSS1和HbSS2)，HbSS1全长2864 bp，编码806个氨基酸；HbSS2全长2815 bp，编码811个氨基酸。两个基因编码的蛋白具有典型的植物蔗糖合成酶结构特征，包含1个磷酸化位点和两个保守的功能域。半定量RT-PCR分析表明，HbSS1和HbSS2在各组织器官中均有表达，其中HbSS1在叶中的表达量最高，HbSS2在树皮中的表达量最高，这说明HbSS1和HbSS2可能参与了各组织的生长和代谢过程，且功能有所分化。