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METHOD OF PREPARING, CLONING AND EXPRESSION OF GENE, GENES, IMMUNOTOXINS, GENE PRODUCTS, DNA, CELL, METHOD OF GENE VERIFICATION AND A METHOD OF MUTATION CORRECTION
METHOD OF PREPARING, CLONING AND EXPRESSION OF GENE, GENES, IMMUNOTOXINS, GENE PRODUCTS, DNA, CELL, METHOD OF GENE VERIFICATION AND A METHOD OF MUTATION CORRECTION
FIELD: molecular biology. SUBSTANCE: invention finds nucleotide sequence of synthetic gene for plant toxin gelonine and a method of preparing, cloning and expression of this synthetic gene. DNA sequence for gelonine synthetic gene is given as sequence ID N 1. Invention proposes also expression vectors containing DNA sequences for gelonine and cells transformed with these vectors. Also, immunotoxin containing antibodies conjugated with protein gelonine is proposed. EFFECT: improved method of procedures.
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机译:领域:分子生物学。发明内容本发明找到植物毒素明胶氨酸合成基因的核苷酸序列,以及该合成基因的制备,克隆和表达方法。用于明胶氨酸合成基因的DNA序列以序列ID N 1给出。本发明还提出了表达载体,其包含用于明胶氨酸的DNA序列和用这些载体转化的细胞。此外,提出了含有与蛋白明胶偶联的免疫毒素的抗体。效果:改进的程序方法。
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