ABSTRACT A novel approach for detecting radiation damage to radiosensitive genes was developed using surface-enhanced Raman scattering (SERS) technology. Radiosensitive gene fragments were modified with thiol groups and immobilized onto the surface of gold nanoparticles (AuNPs) to form single-stranded and double-stranded DNA through Au?S bonds. SERS was used to analyze the response of DNA-AuNPs assemblies to γ-rays and the local enhancement effect of AuNPs on this response to γ-radiation. The results showed that the 663 cm?1 spectral peak of the double-stranded DNA-AuNPs assemblies varied with the dose range of γ-radiation (0 to ~200 Gy). Similarly, the single-stranded DNA-AuNPs showed a good response to γ-radiation in the dose range of 0~100 Gy, and the sensitivity of the DNA-AuNPs assembly noticeably increased with the increase in bound AuNPs. This positive correlation was due to the localized surface plasmon resonance of the AuNPs. The analysis of DNA-AuNPs assemblies using SERS scattering, could potentially be a novel method for the detection and measurement of ionizing radiation causing damage to genes.%本文旨在发展一种基于辐射敏感基因和表面增强拉曼散射(Surface enhanced Raman scattering, SERS)光谱技术的辐射探测方法.通过对辐射敏感基因片段进行巯基修饰,将其以Au?S共价键偶联到金纳米粒子(Gold nanoparticles,AuNPs)表面,形成单链和双链两种DNA-AuNPs组装体.利用SERS技术研究DNA-AuNPs组装体对γ射线的辐射响应性质,以及金纳米粒子的局域增强效应对组装体γ射线响应的影响.结果表明:吸收剂量为0~200 Gy时,双链DNA-AuNPs组装体在663 cm?1处的拉曼峰有明显变化,表现出对吸收剂量的响应性;吸收剂量为0~100 Gy时,单链DNA-AuNPs组装体同样呈现辐射响应性质,且辐射响应灵敏度随着组装体纳米金浓度的增加而增加,这可归因于AuNPs的局域等离激元的增强效应.结果提示,DNA-AuNPs组装体和SERS光谱技术相结合,有望成为一种用于电离辐射剂量测量的新型探测方法.
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