Emdogain作用下人牙周膜细胞的差异表达基因

摘要

Objective:To study the different gene expressions in human periodontal cells after being treated with Emdogain(EMD). Methods: The fourth passage periodontal cells were cultured. Cells in experimental group were cultured with 100 μg/ml EMD in non-serum DMEM for 3 days. Cells in control group were cultured in non-serum DMEM for 3 days. The gene expressions in human periodontal cells were screened with gene chip technique. Results: There were 112 differently expressed genes, 11 down-regulated genes and 101 up-regulated genes. Conclusion: After treated with Emdogain, expression of genes related to cell growth, cell proliferation, biosynthesis of extracellular matrix, angiogenesis and osteogenesis is up-regulated dramatically. Genes related to lipid metabolization and keratinocyte growth is down-regulated in human periodontal cells.%目的:筛选猪釉基质蛋白(EMD,Emdogain)作用于人牙周膜细胞后的差异表达基因.方法:培养第4 代牙周膜细胞,实验组加入终浓度为100 μg/ml EMD的无血清DMEM培养液,对照组为不含EMD的无血清DMEM培养液,培养3 d,采用基因芯片技术检测相关的差异表达基因.结果:共得到112 条差异表达基因,上调基因101 条,下调基因11 条.结论:EMD作用于人牙周膜细胞后,与细胞的生长、增殖,细胞外基质的合成,新生血管的形成,骨形成等功能相关的基因表达都显著提高,而脂蛋白代谢相关基因和角化细胞生长相关基因的表达下调.