目的:探究失交感神经支配对大鼠牵张成骨骨痂内趋化因子PDGF表达的影响.方法:雄性SD大鼠18只随机分为对照组和实验组(n=9),对照组行右侧下颌骨牵张成骨术；实验组行右侧下颌骨牵张成骨术及双侧颈交感干离断术.术后分别于固定期1、14、28 d每组取材3只动物进行免疫组织化学染色评价PDGF的表达情况,并运用IPP6.0软件分析骨小梁周围、未成骨区域及血管内皮细胞周围平均吸光度值,所得结果用SPSS 13.0进行两样本t检验.结果:实验组骨小梁周围、未成骨区域PDGF表达量高于对照组(P＜0.05),而血管内皮细胞周围PDGF表达量明显低于对照组(P＜0.05).结论:在牵张成骨中,PDGF在血管周围和成骨区表达具有时空变化受到交感神经的调控.%Objective: To investigate the effects of sympathetic innervation loss on the expression of chemokine PDGF in bone callus in rat mandibular distraction osteogenesis. Methods: 18 male SD rats were divided into control and experimental groups (re =9) randomly. The rats in control group underwent right mandibular distraction osteogenesis, those in experimental group underwent right mandibular distraction osteogenesis and then bilateral transection of cervical sympathetic trunk. 3 rats were sacrificed at consolidation time of 1,14 and 28th day respectively in each group, and specimens were prepared for immunohistochemical staining of PDGF. IPP6.0 was used for the analysis of average optical density values around trabecular bone and vascular endothelial cells and none ossification area. A two-sample t test was performed using SPSS 13.0 software package. Results: PDGF expression around trabecular bone and none ossification area in experimental group was significantly higher than that in control group (P <0.05) , while PDGF expression around vascular endothelial cells in experimental group was significantly lower than that in control group(P <0. 05). Conclusion: Sympathetic innervation regulates the expression and distribution of PDGF around perivascular area and ossification area in distraction osteogenesis.
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