目的：利用组织芯片技术探讨肺癌组织中Raf激酶抑制蛋白（raf kinase inhibitor protein，RKIP）和磷酸化细胞外信号调节激酶（phosphorylation of extracelluar signal-regulated kinase，p-ERK）的表达及其与临床病理特征的关系。方法：应用免疫组织化学方法检测96例肺癌及相应癌旁正常肺组织中RKIP与p-ERK蛋白的表达情况，并分析其与肺癌临床病理学特征的关系。结果：肺癌组织中RKIP的表达低于癌旁正常肺组织，而p-ERK的表达高于癌旁正常肺组织，差异有统计学意义。其表达水平与肺癌的分化程度、TNM分期、淋巴结转移及术后生存时间有明显关系；RKIP蛋白的表达水平与p-ERK呈负相关（P＜0．05）。结论：RKIP低表达与肺癌的侵袭、转移密切相关，RKIP可能是肺癌的一个转移抑制蛋白，RKIP表达下调导致p-ERK的过表达从而促进肺癌细胞的侵袭和转移。%Objective To investigate the expression of raf kinase inhibitor protein (RKIP) and phospho-rylation of extracelluar signal-regulated kinase (p-ERK) in lung carcinoma by tissue microarray technique, and to explore its correlation with the clinicopathologic features. Methods RKIP and p-ERK protein expression were detected by using immunohistochemistry in 96 lung carcinoma specimens and 96 non-tumor lung samples. Relationship between RKIP and p-ERK expression and clinicopathological parameters was also analyzed. Results Immunohistochemistry assay revealed that RKIP level was lower in tumor tissues than that in normal lung tissues. But p-ERK positive staining rate was significantly higher in tumor tissues than that in normal lung tissues. The difference was significant (P<0.05). RKIP and p-ERK expression was correlated with the different differentiation of tumor, TNM staging, the development of lymph node metastasis and the survival time (P < 0.05, respectively). RKIP expression was negatively correlated with ERK activation in lung carcinoma (P < 0.05). Conclusion Expression of RKIP and p-ERK was significantly correlated with invasion and metastasis of lung carcinoma. RKIP may be a lung carcinoma metastasis suppressor, and the decreased RKIP expression was associated with the increased metastasis capability of lung carcinoma cells through activating ERK.
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